Overexpression of
| Autor: | Saowarath Jantaro, Kamonchanock Eungrasamee, Aran Incharoensakdi, Peter Lindblad |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
FFA secretion
QH301-705.5 Mutant Cellular homeostasis Synechocystis sp. PCC 6803 Fatty Acids Nonesterified Catalysis Article Inorganic Chemistry Membrane Lipids acyl-ACP synthetase Bacterial Proteins Secretion Physical and Theoretical Chemistry Lipase Biology (General) Molecular Biology QD1-999 Spectroscopy chemistry.chemical_classification biology lipase A Lipogenesis Organic Chemistry Synechocystis Wild type Biochemistry and Molecular Biology Fatty acid General Medicine biology.organism_classification Computer Science Applications Chemistry chemistry Biochemistry membrane lipid degradation Mutation biology.protein lipids (amino acids peptides and proteins) Intracellular Biokemi och molekylärbiologi |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 21 International Journal of Molecular Sciences, Vol 22, Iss 11468, p 11468 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Although engineered cyanobacteria for the production of lipids and fatty acids (FAs) are intelligently used as sustainable biofuel resources, intracellularly overproduced FAs disturb cellular homeostasis and eventually generate lethal toxicity. In order to improve their production by enhancing FFAs secretion into a medium, we constructed three engineered Synechocystis 6803 strains including KA (a mutant lacking the aas gene), KAOL (KA overexpressing lipA, encoding lipase A in membrane lipid hydrolysis), and KAOGR (KA overexpressing quadruple glpD/rbcLXS, related to the CBB cycle). Certain contents of intracellular lipids and secreted FFAs of all engineered strains were higher than those of the wild type. Remarkably, the KAOL strain attained the highest level of secreted FFAs by about 21.9%w/DCW at day 5 of normal BG11 cultivation, with a higher growth rate and shorter doubling time. TEM images provided crucial evidence on the morphological changes of the KAOL strain, which accumulated abundant droplets on regions of thylakoid membranes throughout the cell when compared with wild type. On the other hand, BG11-N condition significantly induced contents of both intracellular lipids and secreted FFAs of the KAOL strain up to 37.2 and 24.5%w/DCW, respectively, within 5 days. Then, for the first time, we shone a spotlight onto the overexpression of lipA in the aas mutant of Synechocystis as another potential strategy to achieve higher FFAs secretion with sustainable growth. |
| Databáze: | OpenAIRE |
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