Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature
| Autor: | Pierre Gounon, Sylviane Robineau, Joëlle Bigay, Bruno Antonny |
|---|---|
| Přispěvatelé: | Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
GTP' Lipid Bilayers Golgi Apparatus Small G Protein Biology Models Biological Coat Protein Complex I 03 medical and health sciences 0302 clinical medicine Animals Lipid bilayer COPII 030304 developmental biology 0303 health sciences Multidisciplinary ADP-Ribosylation Factors Hydrolysis Bilayer Vesicle GTPase-Activating Proteins Intracellular Membranes COPI Lipid Metabolism Lipids Cell biology Kinetics Coatomer Liposomes Guanosine Triphosphate Rabbits COP-Coated Vesicles 030217 neurology & neurosurgery |
| Zdroj: | Nature Nature, Nature Publishing Group, 2003, 426, pp.563-566 |
| ISSN: | 0028-0836 1476-4679 |
| Popis: | Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles1,2,3. The assembly/disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP/GDP cycle of the small G protein Arf1. At the heart of this coupling is the specific interaction of membrane-bound Arf1–GTP with coatomer, a complex of seven proteins that forms the building unit of the COPI coat4,5,6,7. Although COPI coat disassembly requires the catalysis of GTP hydrolysis in Arf1 by a specific GTPase-activating protein (ArfGAP1)8,9,10, the precise timing of this reaction during COPI vesicle formation is not known. Using time-resolved assays for COPI dynamics on liposomes of controlled size, we show that the rate of ArfGAP1-catalysed GTP hydrolysis in Arf1 and the rate of COPI disassembly increase over two orders of magnitude as the curvature of the lipid bilayer increases and approaches that of a typical transport vesicle. This leads to a model for COPI dynamics in which GTP hydrolysis in Arf1 is organized temporally and spatially according to the changes in lipid packing induced by the coat. |
| Databáze: | OpenAIRE |
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