Detection of Mycobacterium avium subsp. paratuberculosis in a sheep flock in Tuscany
| Autor: | Barbara Turchi, Filippo Fratini, Alessia Galiero, Domenico Cerri, Giuseppe Colombani, Roberta Nuvoloni |
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| Rok vydání: | 2015 |
| Předmět: |
Genotyping
Immunodiffusion Veterinary medicine Sheeps Genotype Culture Population Sheep Diseases Paratuberculosis Spleen Biology Sheeps Mycobacterium avium subsp. paratuberculosis Garfagnana district Culture Polymerase chain reaction Genotyping Garfagnana district Polymerase Chain Reaction law.invention Feces Food Animals Cheese Ileum law medicine Animals Mesenteric lymph nodes education Sheep Domestic Polymerase chain reaction education.field_of_study Sheep food and beverages medicine.disease Sheep flock Mycobacterium avium subsp. paratuberculosis Milk medicine.anatomical_structure Italy Animal Science and Zoology Lymph Nodes Lymph |
| Zdroj: | Tropical Animal Health and Production. 47:1567-1571 |
| ISSN: | 1573-7438 0049-4747 |
| DOI: | 10.1007/s11250-015-0899-z |
| Popis: | Paratuberculosis is an infectious disease which affects ruminants. In this work, a sheep flock in Garfagnana district (Tuscany, Italy) was examined by agar gel immune-diffusion (AGID) tests, culture, and PCR from feces, milk, tissue samples, and cheeses. At the first AGID test, 7/280 (2.5 %) animals were positive. From these animals, feces and milk samples were collected: 4/7 feces (57.14 %) and 2/7 milk samples (28.57 %) were positive to culture and 7/7 (100 %) feces samples and 1/7 milk samples (14.28 %) were positive to PCR; 2/4 (50 %) cheeses ripened for 14 days and 1/3 (33.33 %) cheese ripened for 20 days were positive to PCR, from which no viable microorganisms were isolated. Then, the AGID-positive animals were slaughtered and tissue samples were taken from one sheep with PCR-positive feces and milk: the liver, intestine, mesenteric lymph nodes, but not the spleen and mammary lymph nodes were positive to culture; all these samples were instead PCR-positive. After 1 year, a second AGID survey was performed on the remaining animals: 6/244 (2.45 %) subjects were positive to this test. Data obtained revealed the presence of paratuberculosis in a sheep population in Garfagnana. |
| Databáze: | OpenAIRE |
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