Supplementation of nano-selenium in fish diet: Impact on selenium assimilation and immune-regulated selenoproteome expression in monosex Nile tilapia (Oreochromis niloticus)
| Autor: | T.G. Puneeth, Shamima Nasren, S. S. Rathore, S.K. Girisha, B.T.N. Kumar, M. A. A. Mamun, M.S. Nithin, K. Rakesh, H.S. Murthy, M. M. Manohara Pai |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Fish Proteins Gills GPX2 food.ingredient Proteome Physiology Health Toxicology and Mutagenesis 0211 other engineering and technologies chemistry.chemical_element Gene Expression 02 engineering and technology Toxicology Kidney Biochemistry 03 medical and health sciences Nile tilapia Selenium food Animal science Animals Tissue Distribution Selenoproteins chemistry.chemical_classification 021110 strategic defence & security studies biology Reverse Transcriptase Polymerase Chain Reaction Muscles Assimilation (biology) Tilapia Cell Biology General Medicine Cichlids biology.organism_classification Animal Feed Diet Nanostructures Oreochromis 030104 developmental biology chemistry Liver Bioaccumulation Dietary Supplements Selenoprotein |
| Zdroj: | Comparative biochemistry and physiology. Toxicologypharmacology : CBP. 240 |
| ISSN: | 1532-0456 |
| Popis: | Selenium (Se), a fundamental element of nutrigenomic science in fish nutrition, was used to investigate its impact on selenoproteome expression and Se regulation in tilapia. Different concentrations (T1 – 0, T2 – 0.5, T3 – 1.0 and T4 – 2.0 mg/kg of feed) of dietary nano-Se were incorporated in the diets of monosex Nile tilapia. A total of 180 tilapia fingerlings with initial weight (15.73 ± 0.05 g) were stocked in 150 L capacity FRP tanks categorized into four diet groups with triplicate each for a feeding trial of 90 days. At the end of first, second and third months of the feeding trial, gill, liver, kidney and muscle tissues were harvested to evaluate the effect on the kinetics of Se bioaccumulation and assimilation as well as immune-regulated selenoprotein transcripts (GPx2, SelJ, SelL, SelK, SelS, SelW and Sepp1a) and their synthesis factors (SPS1 and Scly). The findings depicted that significantly (p |
| Databáze: | OpenAIRE |
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