Molecular detection and phylogenetic analysis of megalocytivirus in Brazilian ornamental fish
| Autor: | Pedro Henrique Magalhães Cardoso, Andrezza Maria Fernandes, Sabrina Ribeiro de Almeida-Queiroz, Simone de Carvalho Balian, Samara Rita de Lucca Maganha, Ricardo Luiz Moro de Sousa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Zoology Aquaculture Megalocytivirus Polymerase Chain Reaction law.invention Fish Diseases 03 medical and health sciences Phylogenetics law Virology Ornamental plant Animals Clade Phylogeny Polymerase chain reaction biology Phylogenetic tree business.industry Fishes General Medicine biology.organism_classification DNA Virus Infections Iridoviridae CITOMEGALOVIRUS 030104 developmental biology Fish Capsid Proteins business Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Megalocytiviruses have a worldwide distribution, causing serious economic loss to the global aquaculture industry. They also present a threat to ornamental fish trade because megalocytiviral infections have unspecified symptoms, making early diagnosis difficult. In this study, 100 ornamental fish from 24 different species were tested by PCR for megalocytivirus, with a 47% positive rate being identified. Phylogenetic reconstruction, based on the major capsid protein (MCP) gene, clustered all Brazilian samples into a single clade, showing identity values ranging from 99% to 100% when compared to each other. This is the first report of megalocytivirus infection in some ornamental fish species in Brazil. |
| Databáze: | OpenAIRE |
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