Immunochemical determination of the serum protein reacting with antibody against human urinary trypsin inhibitor by single radial immunodiffusion: use of polyethylene glycol
| Autor: | Koji Sasaki, Hiroyuki Sumi, Susumu Maehara, Yasutoshi Himeno, Tomoyuki Ishibe, Eiso Kawashita, Naotika Toki, Yoshiaki Tanaka |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Radial immunodiffusion
Immunodiffusion biology Chemistry Trypsin inhibitor Immunology Temperature Polyethylene glycol Urine Blood Proteins Precipitin Trypsin Molecular biology Precipitin Tests Antibodies Polyethylene Glycols Agar plate chemistry.chemical_compound biology.protein medicine Immunology and Allergy Humans Antibody Trypsin Inhibitors medicine.drug |
| Zdroj: | Journal of immunological methods. 80(1) |
| ISSN: | 0022-1759 |
| Popis: | A single radial immunodiffusion method is described for determining the serum protein which reacts with antibody against the urinary trypsin inhibitor, but does not react with anti-inter-alpha-trypsin inhibitor antibody. Since the amount of this protein could not be determined with an agar plate containing antibody alone, we first prepared an agar plate containing 4% polyethylene glycol 6 000 and 1 mg of anti-urinary trypsin inhibitor gamma-globulin and confirmed that the amount of this protein could be measured accurately from the sizes of precipitin rings after 72 h incubation at room temperature. Levels of this serum protein, alpha 1-antitrypsin, alpha 2-macroglobulin and inter-alpha-trypsin inhibitor were measured in normal human serum by the single radial immunodiffusion method, and it was confirmed that the level of this serum protein did not correlate with the levels of these 3 other proteins. |
| Databáze: | OpenAIRE |
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