Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

Autor: Tie Zhou, Jiang Xiong, Feifei Li, Lian Liu, Xuefei Lian, Chaolan Tang, Biwen Lü, Yuanyuan Chang, Xiao-Chuan Ma, Mengjing Tang, Shenxi Xie, Xiaopeng Lu, Zehang Li
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Chloroplasts
Nicotiana benthamiana
Poncirus trifoliata
allene oxide synthase
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Gene Expression Regulation
Plant
Promoter Regions
Genetic
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
biology
Chemistry
Jasmonic acid
General Medicine
Plants
Genetically Modified
Droughts
Computer Science Applications
Trifoliate orange
Cell biology
Intramolecular Oxidoreductases
Chloroplast
Signal transduction
interaction
Cyclopentanes
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Stress
Physiological
Poncirus
Luciferase
Oxylipins
Physical and Theoretical Chemistry
Molecular Biology
Gene
Transcription factor
promoter
Organic Chemistry
biology.organism_classification
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Reactive Oxygen Species
Transcription Factors
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 13
International Journal of Molecular Sciences, Vol 21, Iss 4705, p 4705 (2020)
ISSN: 1422-0067
Popis: Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P&minus
532 (&minus
532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P&minus
532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.
Databáze: OpenAIRE
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