Electrochemiluminescence Microscopy of Cells: Essential Role of Surface Regeneration
| Autor: | Dongni Han, Danjun Fang, Dechen Jiang, Neso Sojic, Bertrand Goudeau |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Chemistry Surface Properties Chinese hamster ovary cell Regeneration (biology) 010401 analytical chemistry Biosensing Techniques CHO Cells Electrochemical Techniques 010402 general chemistry Photochemistry Electrochemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Membrane Cricetulus Electrode Microscopy Luminescent Measurements Luminophore Electrochemiluminescence Animals Cells Cultured |
| Zdroj: | Analytical chemistry. 93(3) |
| ISSN: | 1520-6882 |
| Popis: | Electrochemiluminescence (ECL) microscopy is successfully applied to image cells, micro-/nano-objects, and electrochemical processes at electrode surfaces. The classic ECL tandem system is composed of the [Ru(bpy)3]2+ luminophore with the very efficient tripropylamine (TPA) coreactant. The dramatic decrease of the ECL signal observed when recording successive ECL images constitutes a key limitation for the development of ECL microscopy. Herein, we investigated the progressive decrease of the ECL signal of Chinese hamster ovary (CHO) cells. The plasma membranes of CHO cells were labeled with a [Ru(bpy)3]2+ derivative, and the ECL images were recorded using the TPA coreactant. We demonstrate that the loss of the ECL signal is related to the electrochemical step because of a progressive lower TPA oxidation current. We tested a cathodic regenerative treatment of the electrode surface, which allowed us to restore the initial TPA oxidation intensity and thus to record a sequence of ECL images without any vanishing of the light signal. The electrochemical approach presented here is an essential step for the development of ECL microscopy of cells. |
| Databáze: | OpenAIRE |
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