GRASP65 and GRASP55 sequentially promote the transport of C-terminal valine-bearing cargos to and through the Golgi complex
| Autor: | Giuseppe Di Tullio, Pierfrancesco Marra, Stefano Bonatti, Galina V. Beznoussenko, Giovanni D'Angelo, Gianluca Martire, Marco Savarese, Luisa Iodice, Libera Prencipe, Maria Antonietta De Matteis |
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| Rok vydání: | 2009 |
| Předmět: |
CD8 Antigens
Recombinant Fusion Proteins PDZ domain Golgi Apparatus Plasma protein binding Golgi reassembly Biology Endoplasmic Reticulum Biochemistry Cell Line symbols.namesake Animals Humans RNA Small Interfering Molecular Biology Secretory pathway Unconventional protein secretion Golgi Matrix Proteins Membrane Proteins Valine Cell Biology Golgi apparatus Transport protein Cell biology Protein Structure Tertiary Protein Transport Membrane Transport Structure Function and Biogenesis Membrane protein symbols Protein Binding |
| Zdroj: | The Journal of biological chemistry. 284(50) |
| ISSN: | 1083-351X |
| Popis: | The Golgi matrix proteins GRASP65 and GRASP55 have recognized roles in maintaining the architecture of the Golgi complex, in mitotic progression and in unconventional protein secretion whereas, surprisingly, they have been shown to be dispensable for the transport of commonly used reporter cargo proteins along the secretory pathway. However, it is becoming increasingly clear that many trafficking machineries operate in a cargo-specific manner, thus we have investigated whether GRASPs may control the trafficking of selected classes of cargo. We have taken into consideration the C-terminal valine-bearing receptors CD8alpha and Frizzled4 that we show bind directly to the PSD95-DlgA-zo-1 (PDZ) domains of GRASP65 and GRASP55. We demonstrate that both GRASPs are needed sequentially for the efficient transport to and through the Golgi complex of these receptors, thus highlighting a novel role for the GRASPs in membrane trafficking. Our results open new perspectives for our understanding of the regulation of surface expression of a class of membrane proteins, and suggests the causal mechanisms of a dominant form of autosomal human familial exudative vitreoretinopathy that arises from the Frizzled4 mutation involving its C-terminal valine. |
| Databáze: | OpenAIRE |
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