The lncRNA male-specific abdominal plays a critical role in Drosophila accessory gland development and male fertility
| Autor: | Elodie Prince, Mariana F. Wolfner, Jessica L. Sitnik, Robert K. Maeda, Yohan Frei, François Karch, Dragan Gligorov |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Central Nervous System Male Cancer Research Organogenesis Oviposition Cell morphology Genome Biochemistry Nervous System Animals Genetically Modified Sexual Behavior Animal 0302 clinical medicine Electrochemistry Medicine and Health Sciences Drosophila Proteins Genetics (clinical) Drosophila Melanogaster Eukaryota Animal Models Non-coding RNA Phenotype Cell biology Nucleic acids Insects Chemistry Phenotypes Experimental Organism Systems Physical Sciences Drosophila Female RNA Long Noncoding Drosophila melanogaster Cellular Structures and Organelles Anatomy Research Article Secondary Cells lcsh:QH426-470 Arthropoda Biology Research and Analysis Methods 03 medical and health sciences Model Organisms microRNA Genetics Animals Molecular Biology Ecology Evolution Behavior and Systematics Biology and life sciences Organisms Cell Biology biology.organism_classification Invertebrates Gene regulation Male accessory gland lcsh:Genetics MicroRNAs 030104 developmental biology Electrochemical Cells Fertility Bithorax complex Vacuoles Mutation Long non-coding RNAs RNA Gene expression 030217 neurology & neurosurgery |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 14, Iss 7, p e1007519 (2018) |
| ISSN: | 1553-7404 1553-7390 |
| Popis: | Although thousands of long non-coding RNAs (lncRNA) have been identified in the genomes of higher eukaryotes, the precise function of most of them is still unclear. Here, we show that a >65 kb, male-specific, lncRNA, called male-specific abdominal (msa) is required for the development of the secondary cells of the Drosophila male accessory gland (AG). msa is transcribed from within the Drosophila bithorax complex and shares much of its sequence with another lncRNA, the iab-8 lncRNA, which is involved in the development of the central nervous system (CNS). Both lncRNAs perform much of their functions via a shared miRNA embedded within their sequences. Loss of msa, or of the miRNA it contains, causes defects in secondary cell morphology and reduces male fertility. Although both lncRNAs express the same miRNA, the phenotype in the secondary cells and the CNS seem to reflect misregulation of different targets in the two tissues. Author summary In many animals, the male seminal fluid induces physiology changes in the mated female that increase a male’s reproductive success. These changes are often referred to as the post-mating response (PMR). In Drosophila, the seminal fluid proteins responsible for generating the PMR are made in a specialized gland, analogous to the mammalian seminal vesicle and prostate, called the accessory gland (AG). In this work, we show that a male-specific, long, non-coding RNA (lncRNA), called msa, plays a critical role in the development and function of this gland, primarily through a microRNA (miRNA) encoded within its sequence. This same miRNA had previously been shown to be expressed in the central nervous system (CNS) via an alternative promoter, where its ability to repress homeotic genes is required for both male and female fertility. Here, we present evidence that the targets of this miRNA in the AG are likely different from those found in the CNS. Thus, the same miRNA seems to have been selected to affect Drosophila fertility through two different mechanisms. Although many non-coding RNAs have now been identified, very few can be shown to have function. Our work highlights a lncRNA that has multiple biological functions, affecting cellular morphology and fertility. |
| Databáze: | OpenAIRE |
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