TRP channels in brown and white adipogenesis from human progenitors

Autor: Asim Shabbir, M Marc van Vijven, M. Shabeer Yassin, Cedric Badowski, Mathilde Koch, Michael Raghunath, Sue-Anne Toh, Alfredo Franco-Obregón, Anna G. Goralczyk
Přispěvatelé: Orthopaedic Biomechanics
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Receptors
Cell Surface/genetics
0301 basic medicine
Adipose Tissue
Brown/metabolism
Cellular differentiation
Calcium Channels/genetics
Mitochondrion
Biochemistry
Transient receptor potential channel
Transient Receptor Potential Channels
Adipose Tissue
Brown
Lipid droplet
Receptors
Uncoupling protein
Protein Isoforms
Streptomycin/adverse effects
Adipogenesis
Cell Differentiation
Anti-Bacterial Agents
White/metabolism
Adipose Tissue
Streptomycin
TRPP3
Biotechnology
TRPM8
Adult
medicine.medical_specialty
Adipose Tissue
White/metabolism
Adipose Tissue
White
Receptors
Cell Surface
Transient Receptor Potential Channels/genetics
Biology
03 medical and health sciences
Young Adult
Internal medicine
Brown/metabolism
Coactivator
Genetics
medicine
Humans
Adipogenesis/physiology
Membrane Proteins/genetics
Molecular Biology
Cell Surface/genetics
Gene Expression Regulation/physiology
Mesenchymal Stem Cells/cytology
Membrane Proteins
Mesenchymal Stem Cells
Anti-Bacterial Agents/adverse effects
Aminoglycoside antibiotics
030104 developmental biology
Endocrinology
Gene Expression Regulation
Calcium Channels
Browning
Zdroj: The FASEB Journal, 31(8), 3251-3266. FASEB
ISSN: 0892-6638
Popis: Transient receptor potential (TRP) channels are polymodal cell sensors responding to diverse stimuli and widely implicated in the developmental programs of numerous tissues. The evidence for an involvement of TRP family members in adipogenesis, however, is scant. We present the first comprehensive expression profile of all known 27 human TRP genes in mesenchymal progenitors cells during white or brown adipogenesis. Using positive trilineage differentiation as an exclusion criterion, TRP polycystic (P)3, and TPR melastatin (M)8 were found to be uniquely adipospecific. Knockdown of TRPP3 repressed the expression of the brown fat signature genes uncoupling protein (UCP)-1 and peroxisome proliferator-activated receptor γ coactivator (PGC)-1α as well as attenuated forskolin-stimulated uncoupled respiration. However, indices of generalized adipogenesis, such as lipid droplet morphology and fatty acid binding protein (FAPB)-4 expression, were not affected, indicating a principal mitochondrial role of TRPP3. Conversely, activating TRPM8 with menthol up-regulated UCP-1 expression and augmented uncoupled respiration predominantly in white adipocytes (browning), whereas streptomycin antagonized TRPM8-mediated calcium entry, downregulated UCP-1 expression, and mitigated uncoupled respiration; menthol was less capable of augmenting uncoupled respiration (thermogenesis) in brown adipocytes. TRPP3 and TRPM8 hence appear to be involved in the priming of mitochondria to perform uncoupled respiration downstream of adenylate cyclase. Our results also underscore the developmental caveats of using antibiotics in adipogenic studies.-Goralczyk, A., van Vijven, M., Koch, M., Badowski, C., Yassin, M. S., Toh, S.-A., Shabbir, A., Franco-Obregón, A., Raghunath, M. TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin.
Databáze: OpenAIRE
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