Age and Environment Influences on Mouse Prion Disease Progression: Behavioral Changes and Morphometry and Stereology of Hippocampal Astrocytes

Autor: Cristovam Wanderley Picanço Diniz, Pedro Fernando da Costa Vasconcelos, R R Reis, L L Sobral, R B de Oliveira, João Bento-Torres, Daniel C. Anthony
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Aging
Pathology
Stereology
Striatum
Hippocampal formation
Hippocampus
Biochemistry
Prion Diseases
Immunoenzyme Techniques
Mice
Immunolabeling
0302 clinical medicine
Young adult
Doen?as Pri?nicas / psicologia
Behavior
Animal
lcsh:Cytology
Brain
General Medicine
Comportamento Animal
Hipocampo / patologia
medicine.anatomical_structure
Disease Progression
Research Article
Astrocyte
C?rebro / patologia
Imuno-Histoqu?mica / m?todos
medicine.medical_specialty
Article Subject
Doen?as Pri?nicas / patologia
Environment
Biology
Meio Ambiente
03 medical and health sciences
Immune system
Astr?citos / patologia
Internal medicine
Envelhecimento / fisiologia
medicine
Modelos Animais de Doen?as
Animals
lcsh:QH573-671
Environmental enrichment
Cell Biology
Disease Models
Animal
030104 developmental biology
Endocrinology
Astrocytes
Progress?o da Doen?a
030217 neurology & neurosurgery
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
Repositório Digital do Instituto Evandro Chagas (Patuá)
Instituto Evandro Chagas (IEC)
instacron:IEC
ISSN: 1942-0994
1942-0900
DOI: 10.1155/2017/4504925
Popis: This study received financial support from Conselho Nacional de Pesquisa (CNPq) (Grant nos. 300203/2010-1 and 471077/2007-0) for Cristovam Wanderley Picanc?o Diniz; Coordena??o de Aperfei?oamento de Pessoal de N??vel Superior (CAPES) (Process no. 99999.001533/2014-02) for Cristovam Wanderley Pican?o Diniz; and Universidade Federal do Para, Edital PROPESP/FADESP, PIAPA 2015. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m PA, Brazil. Universidade do Estado do Par?. Centro de Ci?ncias da Sa?de. Bel?m, PA, Brazil. Federal University of Rio de Janeiro. Health Sciences Center. Biomedical Sciences Institute. Vertebrate Embryology Laboratory. Rio de Janeiro, RJ, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m PA, Brazil. University of Oxford. Department of Pharmacology. Lab of Experimental Neuropathology. Oxford, UK. Because enriched environment (EE) and exercise increase and aging decreases immune response, we hypothesized that environmental enrichment and aging will, respectively, delay and increase prion disease progression. Mice dorsal striatum received bilateral stereotaxic intracerebral injections of normal or ME7 prion infected mouse brain homogenates. After behavior analysis, animals were euthanized and their brains processed for astrocyte GFAP immunolabeling. Our analysis related to the environmental influence are limited to young adult mice, whereas age influence refers to aged mice raised on standard cages. Burrowing activity began to reduce in ME7-SE two weeks before ME7-EE, while no changes were apparent in ME7 aged mice (ME7-A). Object placement recognition was impaired in ME7-SE, NBH-A, and ME7-A but normal in all other groups. Object identity recognition was impaired in ME7-A. Cluster analysis revealed two morphological families of astrocytes in NBH-SE animals, three in NBH-A and ME7-A, and four in NBH-EE, ME7-SE, and ME7-EE. As compared with control groups, astrocytes from DG and CA3 priondiseased animals show significant numerical and morphological differences and environmental enrichment did not reverse these changes but induced different morphological changes in GFAP+ hippocampal astroglia. We suggest that environmental enrichment and aging delayed hippocampal-dependent behavioral and neuropathological signs of disease progression.
Databáze: OpenAIRE
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