DNAzymes for amine and peptide lysine acylation

Autor:	Scott K. Silverman, Tianjiong Yao, Jack J. Przybyla, Benjamin M. Brandsen, Peter Yeh, Hannah J. Nilsson, Austin M. Woodard
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Acylation Lysine Aryl Organic Chemistry Deoxyribozyme Peptide DNA Catalytic Biochemistry Combinatorial chemistry Article chemistry.chemical_compound chemistry Biocatalysis Electrophile Amine gas treating Amines Physical and Theoretical Chemistry Peptides
Zdroj:	Org Biomol Chem
ISSN:	1477-0539 1477-0520
DOI:	10.1039/d0ob02015j
Popis:	DNAzymes were previously identified by in vitro selection for a variety of chemical reactions, including several biologically relevant peptide modifications. However, finding DNAzymes for peptide lysine acylation is a substantial challenge. By using suitably reactive aryl ester acyl donors as the electrophiles, here we used in vitro selection to identify DNAzymes that acylate amines, including lysine side chains of DNA-anchored peptides. Some of the DNAzymes can transfer a small glutaryl group to an amino group. These results expand the scope of DNAzyme catalysis and suggest the future broader applicability of DNAzymes for sequence-selective lysine acylation of peptide and protein substrates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::805de31ac2ff3438a753cd98ae33f52b https://doi.org/10.1039/d0ob02015j Zobrazit plný text záznamu