Rapid effects of lipopolysaccharides on indocyanine green clearance in rat liver
| Autor: | Jürgen Schölmerich, Stefan Grüne, Daniela Schinharl, Matthias Michl, Michael Reng, E. Frick, Axel Holstege |
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| Rok vydání: | 2000 |
| Předmět: |
Indocyanine Green
Lipopolysaccharides Male medicine.medical_specialty genetic structures Lipopolysaccharide Kupffer Cells Gadolinium Sepsis chemistry.chemical_compound Phagocytosis Internal medicine Animals Medicine Rats Wistar Cyclic GMP Cells Cultured chemistry.chemical_classification Dose-Response Relationship Drug Hepatology business.industry Gastroenterology medicine.disease eye diseases Rats Oxygen tension Enzyme Endocrinology Liver chemistry Mechanism of action Organic anion transport medicine.symptom business Perfusion Indocyanine green |
| Zdroj: | European Journal of Gastroenterology & Hepatology. 12:679-685 |
| ISSN: | 0954-691X |
| DOI: | 10.1097/00042737-200012060-00018 |
| Popis: | Background Lipopolysaccharides (LPSs) are thought to be one of the triggers of organ reactions to sepsis, which causes hepatocellular dysfunction. This dysfunction can be demonstrated by a reduction of organic anion transport The aim of our study was to assess whether the transport of indocyanine green (ICG) is affected by LPS, and whether Kupffer cells are involved. Methods Single-pass liver perfusion with ICG at a concentration of 57.8 mg/kg/min was performed for 130 min. pH, oxygen tension and perfusion pressure were continuously measured in influent and effluent. Taurocholate was infused at 48.3 mg/kg/min to achieve a stable bile flow. LPS was added at concentrations of 0.45, 0.9 and 1.44 mg/kg/min for 30 min. ICG was determined photometrically in perfusate and bile. To depress the function of Kupffer cells male Wistar rats were treated with GdCl 3 24 h in advance. Primary cultured hepatocytes were used for studying the direct effect of LPS on the uptake rate of ICG. Results Forty-five minutes after administration of LPS a significant dose-dependent decrease of ICG uptake was seen in animals treated with LPS. Livers of animals pretreated with GdCl 3 did not show this decrease. LPS had no direct effect on the uptake of ICG into primary cultured hepatocytes, whereas treatment of these cells with 8-bromo-cGMP resulted in a significant increase of ICG uptake. Conclusion LPS has a rapid dose-dependent effect on the detoxification properties of the liver for ICG. The rapid effect of LPS on ICG uptake in hepatocytes is mediated by Kupffer cells. |
| Databáze: | OpenAIRE |
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