Diagnosis of acute toxoplasmosis by an enzyme immunoassay for specific immunoglobulin m antibodies
| Autor: | H Williams, W. Duermeyer, E H van Elven, A W Joss, H. Van Gruijthuijsen, L Skinner, F. Wielaard |
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| Rok vydání: | 1983 |
| Předmět: |
Microbiology (medical)
Anti-nuclear antibody Acquired Toxoplasmosis Enzyme-Linked Immunosorbent Assay Antibody Specificity medicine Humans chemistry.chemical_classification biology medicine.diagnostic_test business.industry Toxoplasma gondii medicine.disease biology.organism_classification Virology Toxoplasmosis Enzyme Immunoglobulin M chemistry Immunoglobulin G Immunoassay Acute Disease Immunology biology.protein Antibody business Toxoplasma Research Article |
| Zdroj: | Journal of Clinical Microbiology. 17:981-987 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A recently developed enzyme-linked immunosorbent assay for detection of immunoglobulin M (IgM) class antibodies to Toxoplasma gondii was evaluated with respect to specificity and sensitivity. By using an antibody capture principle and F(ab')2 conjugates, interference of rheumatoid factors was absent. No cross-reactions with anti-toxoplasma IgG occurred, and no interference with antinuclear antibodies was found. A large-scale study with about 1,500 clinical specimens revealed a 100% specificity. By testing 79 sera from patients with acute-phase acquired toxoplasmosis, sensitivity was found to be 97%. In routine clinical practice, the IgM-enzyme-linked immunosorbent assay proved to be a more sensitive tool for diagnosis than the immunofluorescent-antibody test. The course of IgM-enzyme-linked immunosorbent assay antibodies in acute patients was studied; IgM reached peak levels within 1 month after onset of illness, and could be demonstrated up to an average of 8 months after onset. |
| Databáze: | OpenAIRE |
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