Removal of area CA3 from hippocampal slices induces postsynaptic plasticity at Schaffer collateral synapses that normalizes CA1 pyramidal cell discharge
| Autor: | Michael R. Uttaro, Rebekah C. Evans, Tiffany Brinkley, Erin M. Sanders, Michele Ferrante, Sarah L. Hawes, Susan N. Wright, Maryam Halavi, Theodore C. Dumas, Carolina Barriga |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Hippocampal formation Plasticity Hippocampus Article 03 medical and health sciences 0302 clinical medicine Postsynaptic potential medicine Animals Rats Long-Evans Receptors AMPA Receptor Neuronal Plasticity Chemistry Pyramidal Cells musculoskeletal neural and ocular physiology General Neuroscience Ca1 pyramidal neuron Excitatory Postsynaptic Potentials Population spike CA3 Region Hippocampal Electric Stimulation 030104 developmental biology medicine.anatomical_structure nervous system Schaffer collateral Synapses Neuroscience 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | Neuroscience Letters. 678:55-61 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2018.05.011 |
| Popis: | Neural networks that undergo acute insults display remarkable reorganization. This injury related plasticity is thought to permit recovery of function in the face of damage that cannot be reversed. Previously, an increase in the transmission strength at Schaffer collateral to CA1 pyramidal cell synapses was observed after long-term activity reduction in organotypic hippocampal slices. Here we report that, following acute preparation of adult rat hippocampal slices and surgical removal of area CA3, input to area CA1 was reduced and Schaffer collateral synapses underwent functional strengthening. This increase in synaptic strength was limited to Schaffer collateral inputs (no alteration to temporoammonic synapses) and acted to normalize postsynaptic discharge, supporting a homeostatic or compensatory response. Short-term plasticity was not altered, but an increase in immunohistochemical labeling of GluA1 subunits was observed in the stratum radiatum (but not stratum moleculare), suggesting increased numbers of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors and a postsynaptic locus of expression. Combined, these data support the idea that, in response to the reduction in presynaptic activity caused by removal of area CA3, Schaffer collateral synapses undergo a relatively rapid increase in functional efficacy likely supported by insertion of more AMPARs, which maintains postsynaptic excitability in CA1 pyramidal neurons. This novel fast compensatory plasticity exhibits properties that would allow it to maintain optimal network activity levels in the hippocampus, a brain structure lauded for its ongoing experience-dependent malleability. |
| Databáze: | OpenAIRE |
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