Enhanced expression of neurotrophic factors by C6 rat glioma cells after transfection with glia maturation factor
| Autor: | Asgar Zaheer, Jessica L. Weiss, Ramon Lim, Pravesh Goyal |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene Expression Regulation
Viral Glia Maturation Factor Neurite Recombinant Fusion Proteins Nerve Tissue Proteins Glia maturation factor Biology Transfection PC12 Cells Adenoviridae Neurotrophic factors medicine Neurites Tumor Cells Cultured Animals Nerve Growth Factors RNA Messenger Brain-derived neurotrophic factor Neurons Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Brain-Derived Neurotrophic Factor Glioma Growth Inhibitors Cell biology Rats Gene Expression Regulation Neoplastic medicine.anatomical_structure Nerve growth factor Lac Operon Cell culture Culture Media Conditioned Immunology biology.protein Neuroglia Neurotrophin |
| Zdroj: | Neuroscience letters. 265(3) |
| ISSN: | 0304-3940 |
| Popis: | Glia maturation factor (GMF) is a 17-kDa protein unique to the nervous system. Although GMF was initially characterized as a growth/differentiation factor, the absence of a leader sequence and its intracellular localization in normal brain suggest an intracellular function as well. In this paper we transfected the C6 glioma cells with GMF cDNA by infecting the cells with a GMF/adenovirus construct. The transfected cells overexpressed GMF but did not secret the protein into the culture medium. However, the transfected cells showed an increased expression of the neurotrophic factors including nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). The increase in neurotrophic activity of the C6 cell conditioned medium was demonstrable by its ability to promote neurite outgrowth in PC12 cells. |
| Databáze: | OpenAIRE |
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