Comparison of repair and rejoining fidelity between two isogenic human ovarian carcinoma cell lines
| Autor: | H. E. Abbott Howley, G. P. Raaphorst |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Time Factors
DNA Repair Cell Survival DNA repair Biology chemistry.chemical_compound Plasmid Ovarian carcinoma Tumor Cells Cultured High doses Humans Radiology Nuclear Medicine and imaging Radiosensitivity Ovarian Neoplasms Cell-Free System Radiological and Ultrasound Technology Carcinoma Dose-Response Relationship Radiation DNA Molecular biology chemistry Cell culture Female Radiation response DNA Damage Plasmids |
| Zdroj: | International Journal of Radiation Biology. 78:1095-1102 |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | The difference in radiosensitivity between two isogenic tumour cell lines was evaluated to determine whether factors such as sublethal and potentially damage repair, DNA double-strand break repair and fidelity of repair can be related to differences in radiosensitivity.The cell lines used were the ovarian carcinoma A2780s and a radiation-resistant derivative A2780cp. Radiation response was measured in terms of cell survival, recovery of sublethal (SLD) and potentially lethal damage (PLD), induction of and recovery of DNA strand breaks, and fidelity of DNA repair using a cell-free plasmid assay.While A2780cp was more resistant to radiation than A2780s, it showed less ability for recovery of SLD and PLD. DNA strand-break induction was the same for both cell lines, while only at very high doses did A2780cp show greater DNA strand-break recovery than A2780s. Fidelity of rejoining DNA was significantly higher in the A2780cp cell line.The difference in radiosensitivity between these two cell lines was not related to recovery of PLD or SLD or to the induction of DNA damage. It appears that fidelity of DNA rejoining, which was significantly higher in the resistant cell line, may be related to the difference in radiosensitivity. |
| Databáze: | OpenAIRE |
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