Red-Near-Infrared Fluorescent Probe for Time-Resolved in Vivo Alkaline Phosphatase Detection with the Assistance of a Photoresponsive Nanocontainer
| Autor: | Weili Wei, Mei Wu, Xu Jie, Haimei Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
In situ Nir light Metal Nanoparticles 010402 general chemistry digestive system 01 natural sciences Analytical Chemistry stomatognathic system In vivo Cell Line Tumor Neoplasms Animals Humans Benzopyrans Yttrium Ytterbium Acetaminophen Fluorescent Dyes Acute liver injury Mice Inbred BALB C Chemistry musculoskeletal neural and ocular physiology 010401 analytical chemistry Near-infrared spectroscopy Optical Imaging Nanocontainer musculoskeletal system Alkaline Phosphatase Fluorescence 0104 chemical sciences Thulium Biophysics Alkaline phosphatase Female Chemical and Drug Induced Liver Injury |
| Zdroj: | Analytical chemistry. 91(20) |
| ISSN: | 1520-6882 |
| Popis: | The monitoring of alkaline phosphatase (ALP) activity in different tissues is significant for disease diagnosis and therapy. However, the time-resolved in vivo sensing of ALP activity remained unresolved. Herein, a novel red-near-infrared fluorescent ALP probe (Cl2-BDCM-ALP) based on a dichloro-substituted dicyanomethylene-4H-chromene derivative was designed and synthesized with high fluorescence efficiency and stability under biological pH range. By using Cl2-BDCM-ALP, ALP activity under an acidic microenvironment such as a tumor site can be sensitively imaged, which cannot be achieved by some previously reported ALP probes. By further loading the Cl2-BDCM-ALP into a near-infrared (NIR) light-responsive nanocontainer, time-resolved long-term imaging of ALP activity was facilely achieved with noninvasive NIR light remote control. Time-resolved variation of ALP activity of the drug-induced acute liver injury mice was successfully monitored in vivo for the first time. This strategy holds great promise in the in situ ALP detection under a broad pH range with temporal resolution. |
| Databáze: | OpenAIRE |
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