Lack of Opsonic Antibody Responses to Invasive Infections With
| Autor: | Anna Bläckberg, Therese de Neergaard, Inga-Maria Frick, Pontus Nordenfelt, Rolf Lood, Magnus Rasmussen |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
bacteraemia Myeloma protein Phagocytosis Microbiology law.invention 03 medical and health sciences Antigen law Medicine antibody responses Opsonin Pathogen 030304 developmental biology Original Research 0303 health sciences biology 030306 microbiology business.industry biology.organism_classification QR1-502 emm type biology.protein Recombinant DNA recurrent infection Antibody business Streptococcus dysgalactiae |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 12 (2021) |
| ISSN: | 1664-302X |
| Popis: | IntroductionStreptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity.Materials and MethodsPatients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function.ResultsSixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells.ConclusionS. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host. |
| Databáze: | OpenAIRE |
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