The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels
| Autor: | Paul T. Seed, Balbir S. Bhogal, K E Harman, Stephen Challacombe, M J Gratian, Martin M. Black |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Enzyme-Linked Immunosorbent Assay
macromolecular substances Dermatology Autoantigens Severity of Illness Index Severity of illness medicine Humans education Pemphigus foliaceus Autoantibodies education.field_of_study Desmoglein 3 business.industry Desmoglein 1 Pemphigus vulgaris Autoantibody IIf Cadherins medicine.disease Pemphigus Immunology Mouth Diseases business Biomarkers |
| Zdroj: | British Journal of Dermatology. 144:775-780 |
| ISSN: | 1365-2133 0007-0963 |
| Popis: | Background Pemphigus vulgaris (PV) and foliaceus (PF) are characterized by antibodies to the desmosomal proteins desmoglein 3 (Dsg3) and desmoglein 1 (Dsg1), respectively. Past studies using indirect immunofluorescence (IIF) as a measure of pemphigus antibody levels have failed to demonstrate consistently a relationship between disease severity and IIF titres. However, IIF is not able to measure separately Dsg1 and 3 antibodies, unlike enzyme-linked immunosorbent assays (ELISA), which utilize recombinant proteins. Objectives To compare independently Dsg1 and 3 antibody levels with the severity of both cutaneous and oral involvement in PV and PF. Patients and methods Four hundred and twenty-four serum samples were analysed from 80 subjects with PV and 24 with PF. IgG antibodies to Dsg1 and 3 were measured by ELISA. For every sample analysed, the associated severity of skin and oral disease were graded from 0 to 3; quiescent, mild, moderate and severe. Results A relationship between Dsg1 antibodies and skin severity was demonstrated such that a 10-unit increase in Dsg1 ELISA value was associated with a 34% chance of having a higher severity score [95% confidence interval (CI), 25–45%, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text