Genome-wide profiling of differentially spliced mRNAs in human fetal cortical tissue exposed to alcohol
| Autor: | Masaaki Torii, Hiroki Morizono, Shahid Mohammad, Anna C. Salzberg, Alexander I. Son, Yuka Imamura Kawasawa, Aiesha Basha, Kazue Hashimoto-Torii |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene isoform Health (social science) Biology Toxicology Biochemistry Article Tissue Culture Techniques 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Pregnancy Gene expression medicine Humans RNA Messenger Gene Maternal-Fetal Exchange Genetics Cerebral Cortex Ethanol Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis RNA Gene Expression Profiling Alternative splicing RNA General Medicine Gene expression profiling Alternative Splicing Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure Neurology Cerebral cortex RNA splicing Female Sequence Alignment 030217 neurology & neurosurgery |
| Zdroj: | Alcohol |
| ISSN: | 1873-6823 |
| Popis: | Excessive alcohol consumption results in significant changes in gene expression and isoforms due to altered mRNA splicing. As such, an intriguing possibility is that disturbances in alternative splicing are involved in key pathological pathways triggered by alcohol exposure. However, no resources have been available to systematically analyze this possibility at a genome-wide scale. Here, we performed RNA sequencing of human fetal cortical slices that were obtained at the late first trimester and exposed to ethanol or in control medium. We report 382 events that were identified as changes affecting the ratio of splicing isoforms in the ethanol-exposed fetal human cortex. Additionally, previously unreported novel isoforms of several genes were also identified. These results provide a broad perspective on the post-transcriptional regulatory network underlying ethanol-induced pathogenesis in the developing human cortex. |
| Databáze: | OpenAIRE |
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