Advanced methods for evaluation of sperm quality
| Autor: | Z. T. Homonnai, Ron Hauser, Joseph B. Lessing, Amnon Botchan, Leah Yogev, Gedalia Paz, Haim Yavetz |
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| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system Urology medicine.medical_treatment Population Semen Fertilization in Vitro Semen analysis Biology Andrology Semen quality fluids and secretions Endocrinology Human fertilization Freezing medicine Humans education education.field_of_study In vitro fertilisation Sperm Count medicine.diagnostic_test urogenital system Osmolar Concentration Sperm washing General Medicine Spermatozoa Sperm Hypotonic Solutions Sperm Motility Female |
| Zdroj: | Andrologia. 27:31-35 |
| ISSN: | 0303-4569 |
| DOI: | 10.1111/j.1439-0272.1995.tb02092.x |
| Popis: | Summary Routine semen analysis includes measurements of sperm concentration, motility, and morphology. In our study, three additional tests were evaluated in relation to fertilization rate in an in vitro fertilization program: the freezing and hypo-osmotic swelling tests that evaluate the functional integrity and stability of the sperm membrane under extreme osmotic conditions, and migration sedimentation test that isolates high-quality motile sperm cells. The study was performed on semen delivered by men of couples treated at the In Vitro Fertilization Unit, and men who served as semen donors (fresh or cryopreserved semen). No correlation was found between the sperm fertilization rate, and the decrease in motility percent following the freezing-thawing process. Thus, the freezing test cannot be used to predict semen fertilization capacity. The hypo-osmotic swelling test was applied on semen given by a similar population of men. While the hypo-osmotic swelling test values carried out with fresh semen was found to have good correlation with fertilization, no correlation was found when frozen thawed semen was used. Post-migration sedimentation test sperm characteristics, and especially the recovery rate of the motile sperm, were significantly better in in vitro fertilization cycles with fertilizations. Both the hypo-osmotic swelling test and migration sedimentation test can assist in evaluating semen quality, judged by the fertilization rate in an in vitro fertilization program. |
| Databáze: | OpenAIRE |
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