Embryonic stem cell-derived neural progenitors transplanted to the hippocampus migrate on host vasculature
| Autor: | Laura Grabel, Sandy Becker, Chelsea M. Lassiter, Julian S. Gal, Nathaniel W. Hartman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Integrin Hippocampus Hippocampal formation 03 medical and health sciences Mice Neural Stem Cells Cell Adhesion Animals Humans Progenitor cell Cell adhesion lcsh:QH301-705.5 Cells Cultured Embryonic Stem Cells Medicine(all) biology Integrin alpha6beta1 Endothelial Cells Cell Differentiation General Medicine Cell Biology Embryonic stem cell Neural stem cell Chemokine CXCL12 Coculture Techniques Cell biology 030104 developmental biology lcsh:Biology (General) Astrocytes Immunology biology.protein Blood Vessels Laminin Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 16, Iss 3, Pp 579-588 (2016) |
| ISSN: | 1876-7753 1873-5061 |
| Popis: | This study describes the migration of transplanted ESNPs either injected directly into the hippocampus of a mouse, seeded onto hippocampal slices, or under in vitro culture conditions. We show that transplanted mouse ESNPs associate with, and appear to migrate on the surface of the vasculature, and that human ESNPs also associate with blood vessels when seeded on hippocampal slices, and migrate towards BECs in vitro using a Boyden chamber assay. This initial adhesion to vessels is mediated, at least in part, via the integrin α6β1, as observed for SVZ neural progenitor cells. Our data are consistent with CXCL12, expressed by the astroglial-vasculature niche, playing an important role in the migration of transplanted neural progenitors within and outside of the hippocampus. |
| Databáze: | OpenAIRE |
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