| Popis: |
Supplementary Figure S2. Cytotoxic activity of NK cells from CD1-Foxn1nu mice. A, The MHC class II-deficient mouse lymphoma cell line, YAC-1, was used to test NK cell cytotoxicity. B, IL-2 (dark gray) or IL-15-activated (light gray) NK cells isolated from the spleen of CD1-Foxn1nu mice were plated with YAC-1 cells at an E:T ratio of 5:1 and 2.5:1 for the duration of 4 hours. Specific tumor lysis was significantly higher with NK cells activated with IL-15 than with IL-2. C, Xenoreactivity of murine NK cells against K562 and SJNBL46_X (PDX) with (+AB) and without antibody compared to YAC-1. D, CD107a expression was increased in IL-2- and IL-15-preactivated NK cells compared to resting NK cells after co-culture with YAC-1. E, To quantitate IFN-γ expression, NK cells were stimulated with IL-12/IL-18 in culture (red). Unstimulated NK cells served as controls (gray). IL-15 preactivation was associated with higher IFN-γ expression than was IL-2 or no preactivation. F, Expression analysis of perforin and granzyme B. The expression of perforin was slightly higher in IL-15 than in IL-2-activated NK cells; however, granzyme B was similar. |
