Formation of additional contacts of chromosome with membrane in the process of DNA repair synthesis in bacterial cells
| Autor: | A. I. Gaziev, Yu. Yu. Malinovsky, Bezlepkin Vg |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
DNA
Bacterial biology DNA Repair Ultraviolet Rays Mutant Cell Membrane DNA replication General Medicine Bacillus subtilis DNA-Directed DNA Polymerase Chromosomes Bacterial biology.organism_classification Molecular biology Applied Microbiology and Biotechnology chemistry.chemical_compound chemistry Biosynthesis biology.protein Genetics Thymidine Replication protein A DNA Polymerase |
| Zdroj: | Zeitschrift für allgemeine Mikrobiologie. 23:607-619 |
| ISSN: | 0044-2208 |
| DOI: | 10.1002/jobm.19830231002 |
| Popis: | An increase in the amount of membrane-bound DNA was found in B. subtilis cells with UV-induced DNA repair synthesis as compared to untreated cells. It was shown that DNA repair synthesis occurred in DNA membrane complexes (DMC) formed during UV-irradiation. UV-induced formation of DMC was observed in cells of wild type strains which were capable of repairing damaged DNA but not in a mutant defective in DNA-polymerase I. It was demonstrated that DNA-polymerase I is located on the membrane of B. subtilis cells. This suggested a participation of DNA-polymerase I in binding of the chromosome to the membrane in UV-irradiated cells. UV-induced DMC did not dissociate when the cells were treated with inhibitors of DNA-gyrase. It, therefore, was qualitatively different from the DMC found during replication. The mechanisms of binding of the damaged DNA to the membrane in UV-irradiated cells of B. subtilis are discussed. |
| Databáze: | OpenAIRE |
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