Sperm nuclei analysis of 1/29 Robertsonian translocation carrier bulls using fl uorescence in situ hybridization
| Autor: | André Eggen, Alain Ducos, HM Berland, B. Khireddine, M Yerle, A. Bonnet-Garnier, Alain Pinton, R. Darre |
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| Přispěvatelé: | Cytogénétique des Populations Animales (CPA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
TRANSLOCATION 1/29 [SDV]Life Sciences [q-bio] CATTLE Robertsonian translocation Biology medicine.disease_cause Translocation Genetic 03 medical and health sciences 0302 clinical medicine FISH Genetics medicine Animals Ejaculation Molecular Biology In Situ Hybridization Fluorescence Genetics (clinical) ComputingMilieux_MISCELLANEOUS 030304 developmental biology Cell Nucleus Chromosome Aberrations 0303 health sciences 030219 obstetrics & reproductive medicine medicine.diagnostic_test Genetic Carrier Screening Spermatozoa Molecular biology Sperm CYTOGENETICS 1/29 TRANSLOCATION [SDV] Life Sciences [q-bio] Fluorescence in situ hybridization SPERM |
| Zdroj: | Cytogenetic and Genome Research Cytogenetic and Genome Research, Karger, 2006, 112, pp.241-247 HAL |
| ISSN: | 1424-8581 1424-859X |
| Popis: | In 1964, Gustavsson and Rockborn first described the 1/29 Robertsonian translocation in cattle. Since then, several studies have demonstrated the negative effect of this particular chromosomal rearrangement on the fertility of carrier animals. During the last decade, meiotic segregation patterns have been studied on human males carrying balanced translocations using FISH on decondensed sperm nuclei. In this work, we have applied the ‘Sperm-FISH’ technique to determine the chromosomal content of spermatozoa from two bulls heterozygous for the 1/29 translocation and one normal bull (control). 5425 and 2702 sperm nuclei were scored, respectively, for the two heterozygous bulls, using whole chromosome painting probes of chromosomes 1 and 29. Very similar proportions of normal (or balanced) spermatozoa resulting from alternate segregation were observed (97.42% and 96.78%). For both heterozygous bulls, the proportions of nullisomic and disomic spermatozoa did not follow the theoretical 1:1 ratio. Indeed, proportions of nullisomic spermatozoa were higher than those of disomic sperma tozoa (1.40% vs 0.09% (bull 1) and 1.29% vs 0.15% (bull 2) for BTA1, and 0.65% vs 0.40% (bull 1) and 1.11% vs 0.63% (bull 2) for BTA29). The average frequencies of disomic and diploid spermatozoa in the normal bull were 0.11% and 0.05%, respectively. |
| Databáze: | OpenAIRE |
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