Real-time monitoring of skin ethanol gas by a high-sensitivity gas phase biosensor (bio-sniffer) for the non-invasive evaluation of volatile blood compounds
| Autor: | Ming Ye, Po-Jen Chien, Takuma Suzuki, Takahiro Arakawa, Kenta Iitani, Koji Toma, Kohji Mitsubayashi, Yasuhiko Iwasaki, Masato Tsujii |
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| Rok vydání: | 2018 |
| Předmět: |
Biomedical Engineering
Biophysics Alcohol Human skin 02 engineering and technology Acetaldehyde Biosensing Techniques Nicotinamide adenine dinucleotide 01 natural sciences Fluorescence chemistry.chemical_compound Limit of Detection Electrochemistry Ultraviolet light Humans Ethanol fuel Alcohol dehydrogenase Skin Volatile Organic Compounds Chromatography Ethanol biology 010401 analytical chemistry Alcohol Dehydrogenase General Medicine Equipment Design 021001 nanoscience & nanotechnology NAD 0104 chemical sciences Kinetics chemistry Breath Tests biology.protein 0210 nano-technology Biosensor Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 129 |
| ISSN: | 1873-4235 |
| Popis: | In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) and real-time monitoring system with skin gas cell was constructed for the determination of ethanol gas concentration on human skin. This bio-sniffer measured the concentration of ethanol according to the change in fluorescence intensity of nicotinamide adenine dinucleotide (NADH), which is produced in an enzymatic reaction by alcohol dehydrogenase (ADH). The NADH detection system used an ultraviolet light emitting diode (UV–LED) as the excitation light, and a highly sensitive photomultiplier tube as a fluorescence intensity detector. The calibration range of the ethanol bio-sniffer was validated from 25 ppb to 128 ppm . To measure the concentration of ethanol within skin gas, subjects ingested an alcohol beverage, and the sensor output was monitored. We chose the central part of the palm, a back of the hand, and a wrist as targets. The real-time concentration of skin ethanol gas at each target was measured after drinking. The maximum output values were reached at approximately 70 min after drinking and then gradually decreased. We showed that ethanol release kinetics were different depending on the part of the hand measured with the developed monitoring system. Accordingly, this highly sensitive and selective bio-sniffer with a skin gas cell could be used to measure ethanol on the skin surface and could be applied for breath and skin gas research, as well as investigation of volatile blood compounds used as biomarkers for clinical diagnosis. |
| Databáze: | OpenAIRE |
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