A proteomic investigation into a human gastric cancer cell line BGC823 treated with diallyl trisulfide
| Autor: | Ningzhi Xu, Rui-fang Guo, Kang Zhao, Youyong Lu, Shuting Li, Na Li, Siqi Liu, Jianmin Shao, Wenmei Li, Xishu Chen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Proteomics
Cancer Research Apoptosis Biology Sulfides Mass Spectrometry chemistry.chemical_compound Stomach Neoplasms Cell Line Tumor Image Processing Computer-Assisted Humans Electrophoresis Gel Two-Dimensional Galectin Annexin A1 Glutathione Transferase Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction food and beverages General Medicine Blot Allyl Compounds Gene Expression Regulation Neoplastic Diallyl trisulfide chemistry Biochemistry Cell culture Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Carcinogenesis. 27(6) |
| ISSN: | 0143-3334 |
| Popis: | Garlic is generally used as a therapeutic reagent against various diseases worldwide. Although a great effort is made to understand the pharmaceutical mechanisms of garlic and its derivatives, there are many mysteries to be uncovered. Using proteomic means, herein we have systematically studied the responses of protein expression in BGC823 cells, a gastric cancer cell line, induced by diallyl trisulfide (DATS), a major component of garlic derivatives. A total of 41 unique proteins in BGC823 were detected with significant changes in their expression levels corresponding with DATS administration. Of these proteins, five typical ones, glutathione S-transferase-pi (GST-pi), voltage-dependent anion channel-1 (VDAC-1), Annexin I, Galectin and S100A11, were further examined by Western blotting, resulting in coincident data with the proteomic evidence. Moreover quantitative real-time RT-PCR experiments offered dynamic data of mRNA expression, indicating the responses of Annexin I and GST-pi expression within a short period after DATS treatment. Interestingly, approximately 50% of DATS-sensitive proteins (19/41) in BGC823 are tightly associated with apoptotic pathways. These proteomic results presented, therefore, provide additional support to the hypothesis that garlic is a strong inducer of apoptosis in tumor cells. |
| Databáze: | OpenAIRE |
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