Preparative HPLC separation of methoxytetralins, ligands for melatonin receptors, containing two chiral centers with polysaccharide chiral stationary phases. Determination of enantiomeric purity
| Autor: | Jean-Paul Bonte, Abdelhalim Guelzim, Emmanuelle Lipka, Saïd Yous, Claude Vaccher |
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| Rok vydání: | 2005 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography Tetrahydronaphthalenes Resolution (mass spectrometry) Chemistry Elution Receptors Melatonin Biophysics Stereoisomerism Ligands Polysaccharide Biochemistry Chiral column chromatography chemistry.chemical_compound Phase (matter) Cellulose Enantiomer Chromatography High Pressure Liquid |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 64:46-58 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/j.jbbm.2005.05.008 |
| Popis: | Preparative chromatography was used to obtain approximately 200 mg of each of the four individual isomers of the three methoxytetrahydronaphthalenic derivatives, new agonist and antagonist ligands for melatonin receptors to be tested for binding. The mobile and stationary phases were chosen to achieve best resolution in shorter runtime. Enantiomeric purity was verified and quantified using normal and reversed phase methodology on cellulose CSPs. Enantiomer elution order was analysed and discussed. Limit of detection (LOD) and limit of quantification (LOQ) were calculated. |
| Databáze: | OpenAIRE |
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