Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study

Autor: Bruno Demé, Laura da Silva, Judith Peters, Francesca Natali, Valerie Laux-Lesourd, Tilo Seydel, David W. Deamer, Giuseppe Zaccai, Marie Christine Maurel, Jacques Ollivier, Michael Haertlein, Loreto Misuraca
Přispěvatelé: Dipartimento di Chimica e Fisica della Terra [Palermo], Università degli studi di Palermo - University of Palermo, Institut Laue-Langevin (ILL), CNR Istituto Officina dei Materiali (IOM), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), University of California [Santa Cruz] (UC Santa Cruz), University of California (UC), ILL, Consiglio Nazionale delle Ricerche [Roma] (CNR), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UCSC), University of California, ILL Grenoble, F-38042 Grenoble, France, Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)-Muséum national d'Histoire naturelle (MNHN), Institut de biologie structurale [1992-2019] (IBS - UMR 5075 [1992-2019]), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
multilamellar lipid matrix
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
neutron scattering
mononucleotide mobility
hydration
Phospholipid
Neutron scattering
010402 general chemistry
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
chemistry.chemical_compound
Molecule
lcsh:Science
Lipid bilayer
Ecology
Evolution
Behavior and Systematics
chemistry.chemical_classification
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Paleontology
Polymer
0104 chemical sciences
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
030104 developmental biology
Membrane
chemistry
Biochemistry
Deuterium
Polymerization
Space and Planetary Science
Chemical physics
lcsh:Q
lipids (amino acids
peptides
and proteins)
Zdroj: Life
Life, 2017, Origin of Cellular Life, 7 (1), pp.2. ⟨10.3390/life7010002⟩
Life; Volume 7; Issue 1; Pages: 2
Life (Basel) 7 (2017). doi:10.3390/life7010002
info:cnr-pdr/source/autori:Misuraca L.; Natali F.; da Silva L.; Peters J.; Deme B.; Ollivier J.; Seydel T.; Laux-Lesourd V.; Haertlein M.; Zaccai G.; Deamer D.; Maurel M.C./titolo:Mobility of a mononucleotide within a lipid matrix: A neutron scattering study/doi:10.3390%2Flife7010002/rivista:Life (Basel)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:7
Life, MDPI, 2017, Origin of Cellular Life, 7 (1), pp.2. ⟨10.3390/life7010002⟩
'Life ', vol: 7, pages: 1-10 (2017)
Life, Vol 7, Iss 1, p 2 (2017)
Life, MDPI, 2017, 7 (1), pii: E2. ⟨10.3390/life7010002⟩
ISSN: 2075-1729
Popis: International audience; An essential question in studies on the origins of life is how nucleic acids were first synthesized and then incorporated into compartments about 4 billion years ago. A recent discovery is that guided polymerization within organizing matrices could promote a non-enzymatic condensation reaction allowing the formation of RNA-like polymers, followed by encapsulation in lipid membranes. Here, we used neutron scattering and deuterium labelling to investigate 5'-adenosine monophosphate (AMP) molecules captured in a multilamellar phospholipid matrix. The aim of the research was to determine and compare how mononucleotides are captured and differently organized within matrices and multilamellar phospholipid structures and to explore the role of water in organizing the system to determine at which level the system becomes sufficiently anhydrous to lock the AMP molecules into an organized structure and initiate ester bond synthesis. Elastic incoherent neutron scattering experiments were thus employed to investigate the changes of the dynamic properties of AMP induced by embedding the molecules within the lipid matrix. The influence of AMP addition to the lipid membrane organization was determined through diffraction measurement, which also helped us to define the best working Q range for dynamical data analysis with respect to specific hydration. The use of different complementary instruments allowed coverage of a wide time-scale domain, from ns to ps, of atomic mean square fluctuations, providing evidence of a well-defined dependence of the AMP dynamics on the hydration level.
Databáze: OpenAIRE
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