Cell-specific and pH-sensitive nanostructure hydrogel based on chitosan as a photosensitizer carrier for selective photodynamic therapy
| Autor: | Simin Belali, Mahnaz Hadizadeh, Ali Reza Karimi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_treatment
Photodynamic therapy 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Biochemistry Chitosan chemistry.chemical_compound Structural Biology Neoplasms medicine Humans Photosensitizer Molecular Biology Drug Carriers Photosensitizing Agents Singlet oxygen technology industry and agriculture Hydrogels Hep G2 Cells General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Porphyrin Combinatorial chemistry 0104 chemical sciences Photochemotherapy chemistry Self-healing hydrogels MCF-7 Cells 0210 nano-technology Drug carrier |
| Zdroj: | International Journal of Biological Macromolecules. 110:437-448 |
| ISSN: | 0141-8130 |
| Popis: | The major problems of porphyrins as promising materials for photodynamic therapy (PDT) are their low solubility, subsequently aggregation in biological environments, and a lack of tumor selectivity. With this in mind, a chitosan-based hydrogel conjugated with tetrakis(4-aminophenyl)porphyrin (NH2-TPP) and 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine (TRIPOD) via Schiff base linkage, functionalized with folate was designed and synthesized as a pH-sensitive, self-healable and injectable targeted PS delivery system. This new hydrogel was characterized by FT-IR, 1H NMR, SEM, UV-vis, fluorescence spectroscopy and zeta potential. Formation of imine bonds with the aldehyde group of TRIPOD and amine group of NH2-TPP and chitosan, as a dynamic connection, was approved by rheological analysis. Spectroscopic characterizations revealed that aggregation of porphyrin in aqueous media was eliminated due to diminished π stacking interaction of porphyrin in 3D cross-linked hydrogel structure. Hydrogel 3D microporous structure efficiently transfers the excitation energy to the porphyrin unit, yielding improvement singlet oxygen releases. Cytotoxicity and phototoxicity analysis of the CS/NH2-TPP/FA hydrogels indicating an excellent capability to kill cancer cells selectively and prevent damage to normal cells. This work presents a new and efficient model for the preparation of highly efficient and targeting photosensitizer delivery system. |
| Databáze: | OpenAIRE |
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