Mechanical properties of the domains of titin in a Go-like model
Autor: | Cieplak M, Pastore A, Hoang TX, RI Trinh Hoang/B-6119-2008 |
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Přispěvatelé: | Cieplak, M, Pastore, A, Hoang, Tx, RI Trinh, Hoang/B-6119-2008 |
Jazyk: | angličtina |
Rok vydání: | 2004 |
Předmět: |
Physics
Tandem biology General Physics and Astronomy FOS: Physical sciences Biomolecules (q-bio.BM) Homology (mathematics) Condensed Matter - Soft Condensed Matter Domain (software engineering) Folding (chemistry) Quantitative Biology - Biomolecules Chemical physics Mechanical stability FOS: Biological sciences biology.protein Intermediate state Soft Condensed Matter (cond-mat.soft) Titin Physical and Theoretical Chemistry Native structure |
Popis: | Comparison of properties of three domains of titin, I1, I27 and I28, in a simple geometry-based model shows that despite a high structural homology between their native states different domains show similar but distinguishable mechanical properties. Folding properties of the separate domains are predicted to be diversified which reflects sensitivity of the kinetics to the details of native structures. The Go-like model corresponding to the experimentally resolved native structure of the I1 domain is found to provide the biggest thermodynamic and mechanical stability compared to the other domains studied here. We analyze elastic, thermodynamic and kinetic properties of several structures corresponding to the I28 domain as obtained through homology-based modeling. We discuss the ability of the models of the I28 domain to reproduce experimental results qualitatively. A strengthening of contacts that involve hydrophobic amino acids does not affect theoretical comparisons of the domains. Tandem linkages of up to five identical or different domains unravel in a serial fashion at low temperatures. We study the nature of the intermediate state that arises in the early stages of the serial unraveling and find it to qualitatively agree with the results of Marszalek et al. 11 pages, 19 figures, to appear in J. Chem. Phys |
Databáze: | OpenAIRE |
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