Maize Thymidine Kinase Activity Is Present throughout Plant Development and Its Heterologous Expression Confers Tolerance to an Organellar DNA-Damaging Agent
| Autor: | Jeannine Drouin-Wahbi, Cécile Raynaud, Jorge M. Vázquez-Ramos, Luis Jiro Suzuri-Hernández, Christelle Mazubert, José Antonio Pedroza-Garcia, Javier Plasencia, Manuela Nájera-Martínez |
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| Přispěvatelé: | Universidad Nacional Autónoma de México (UNAM), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Thymidine kinase activity Plastid localization [SDV]Life Sciences [q-bio] Mutant Arabidopsis Plant Science nucleotide metabolism Zea mays 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound thymidine kinase Arabidopsis thaliana Thymidine kinase 1 Ecology Evolution Behavior and Systematics Thymidine monophosphate Ecology biology Botany food and beverages biology.organism_classification Cell biology 030104 developmental biology chemistry Thymidine kinase QK1-989 DNA damage 010606 plant biology & botany |
| Zdroj: | Plants Plants, MDPI, 2020, 9 (8), pp.930. ⟨10.3390/plants9080930⟩ Volume 9 Issue 8 Plants, 2020, 9 (8), pp.930. ⟨10.3390/plants9080930⟩ Plants, Vol 9, Iss 930, p 930 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9080930 |
| Popis: | Thymidine kinase 1 (TK1) phosphorylates thymidine nucleosides to generate thymidine monophosphate. This reaction belongs to the pyrimidine salvage route that is phylogenetically conserved. In the model plant Arabidopsis thaliana, TK activity contributes to maintain nuclear and organellar genome integrity by providing deoxythymidine-triphosphate (dTTP) for DNA synthesis. Arabidopsis has two TK1 genes (TK1a and TK1b) and double mutants show an albino phenotype and develop poorly. In contrast, maize (Zea mays L.) has a single TK1 (ZmTK1) gene and mutant plants are albino and display reduced genome copy number in chloroplasts. We studied the role of ZmTK1 during development and genotoxic stress response by assessing its activity at different developmental stages and by complementing Arabidopsis tk1 mutants. We found that ZmTK1 transcripts and activity are present during germination and throughout maize development. We show that ZmTK1 translocation to chloroplasts depends on a 72-amino-acid N-signal and its plastid localization is consistent with its ability to complement Arabidopsis tk1b mutants which are hypersensitive to ciprofloxacin (CIP), a genotoxic agent to organellar DNA. Also, ZmTK1 partly complemented the Arabidopsis double mutant plants during development. Our results contribute to the understanding of TK1 function in monocot species as an organellar enzyme for genome replication and repair. |
| Databáze: | OpenAIRE |
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