Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii
| Autor: | Alexey V. Tkach, Ashley R. Murray, Anna A. Shvedova, Bengt Fadeel, Susanna Kumlien Georén, Annika Scheynius, Britta Andersson-Willman, Mikael C. I. Karlsson, Yunying Chen, Antonio Barragan, Romanico B. G. Arrighi, Linda Swedin |
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| Rok vydání: | 2012 |
| Předmět: |
Pulmonary Fibrosis
Health Toxicology and Mutagenesis Toxicology Dendritic cells Mice 0302 clinical medicine Pulmonary fibrosis Lung Immunity Cellular 0303 health sciences medicine.diagnostic_test Toxoplasma gondii General Medicine 3. Good health medicine.anatomical_structure medicine.symptom Bronchoalveolar Lavage Fluid Toxoplasma Lung and spleen immunohistology lcsh:Industrial hygiene. Industrial welfare Carbon nanotubes Inflammation Spleen Biology 03 medical and health sciences Immune system lcsh:RA1190-1270 parasitic diseases Intubation Intratracheal medicine Splenocyte Animals lcsh:Toxicology. Poisons Bioluminescence imaging 030304 developmental biology Nanotubes Carbon Research Macrophages Pneumonia medicine.disease biology.organism_classification Mice Inbred C57BL Toxoplasmosis Animal Bronchoalveolar lavage Immunology lcsh:HD7260-7780.8 Inflammation markers 030215 immunology |
| Zdroj: | Particle and Fibre Toxicology Particle and Fibre Toxicology, Vol 9, Iss 1, p 16 (2012) |
| ISSN: | 1743-8977 |
| DOI: | 10.1186/1743-8977-9-16 |
| Popis: | Background Single-walled carbon nanotubes (SWCNT) trigger pronounced inflammation and fibrosis in the lungs of mice following administration via pharyngeal aspiration or inhalation. Human exposure to SWCNT in an occupational setting may occur in conjunction with infections and this could yield enhanced or suppressed responses to the offending agent. Here, we studied whether the sequential exposure to SWCNT via pharyngeal aspiration and infection of mice with the ubiquitous intracellular parasite Toxoplasma gondii would impact on the immune response of the host against the parasite. Methods C57BL/6 mice were pre-exposed by pharyngeal administration of SWCNT (80 + 80 μg/mouse) for two consecutive days followed by intravenous injection with either 1x103 or 1x104 green fluorescence protein and luciferase-expressing T. gondii tachyzoites. The dissemination of T. gondii was monitored by in vivo bioluminescence imaging in real time for 7 days and by plaque formation. The inflammatory response was analysed in bronchoalveolar lavage (BAL) fluid, and by assessment of morphological changes and immune responses in lung and spleen. Results There were no differences in parasite distribution between mice only inoculated with T. gondii or those mice pre-exposed for 2 days to SWCNT before parasite inoculum. Lung and spleen histology and inflammation markers in BAL fluid reflected the effects of SWCNT exposure and T. gondii injection, respectively. We also noted that CD11c positive dendritic cells but not F4/80 positive macrophages retained SWCNT in the lungs 9 days after pharyngeal aspiration. However, co-localization of T. gondii with CD11c or F4/80 positive cells could not be observed in lungs or spleen. Pre-exposure to SWCNT did not affect the splenocyte response to T. gondii. Conclusions Taken together, our data indicate that pre-exposure to SWCNT does not enhance or suppress the early immune response to T. gondii in mice. |
| Databáze: | OpenAIRE |
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