Reduction of the leukemogenic potential of malignant murine leukemic cells by in vivo treatment with recombinant human granulocyte colony-stimulating factor
| Autor: | Tetsuro Orita, Masayoshi Ono, Masakazu Hasegawa, Taira Maekawa, Masahiko Tamura, Hitoshi Nomura, Masayoshi Oh-eda, Tatsuo Abe |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Cancer Research medicine.medical_treatment Mice Inbred Strains Spleen CHO Cells Biology Leukemogenic Mice In vivo Cricetinae Granulocyte Colony-Stimulating Factor Tumor Cells Cultured medicine Animals Humans Clonogenic assay Chromosome Aberrations fungi Granulocyte-Macrophage Colony-Stimulating Factor Hematology Immunotherapy Recombinant Proteins Granulocyte colony-stimulating factor Cytokine medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Oncology Leukemia Myeloid Karyotyping Immunology Cancer research Cell Division Neoplasm Transplantation medicine.drug |
| Zdroj: | Leukemia Research. 17:593-600 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/0145-2126(93)90090-8 |
| Popis: | We have investigated the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) administration on the leukemogenic potential of L-103 murine leukemic cells. Leukemogenic potential was assessed by comparing the regression lines drawn between the number of inoculated leukemic spleen cells and the mean survival time (MST) of the syngeneic recipients. rhG-CSF injected 2.5 μg daily for 14 days reduced the leukemogenic potential of spleen cells of the leukemic mice to 1/200 of the control. This phenomenon was not observed with the leukemic spleen cells treated with r-murine granulocyte-macrophage (rmGM)-CSF in vivo. Cytochemical study indicated that morphologically identifiable blast cells were fewer in the rhG-CSF-treated leukemic spleen. Furthermore, leukemic cells in the rhG-CSF-treated spleen were less proliferative than the control in spite of having more clonogenic cells in the leukemic cell preparation. Cytogenetical analysis showed that chromosome abnormalities found in the original leukemic cells were not altered by rhG-CSF administrations. It also showed that the frequency of the abnormal karyotype was reduced in rhG-CSF-treated leukemic spleen ( 4 17 ) as compared with the control ( 8 8 ), indicating that the mitotic fraction was smaller in the rhG-CSF-treated leukemic cells. These findings indicate that in addition to the reduced number of leukemic cells in the spleen cell preparation, a reduction of the proliferative capacity of the original leukemic cells is involved in the reduction of leukemogenic potential of leukemic cells treated with rhG-CSF in vivo. |
| Databáze: | OpenAIRE |
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