Tetanus Toxoid carrier protein induced T-helper cell responses upon vaccination of middle-aged adults

Autor: Annemieke M. H. Boots, Marieke van der Heiden, Anne-Marie Buisman, Aafke Duizendstra, Guy A. M. Berbers
Přispěvatelé: Translational Immunology Groningen (TRIGR), Microbes in Health and Disease (MHD)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Middle-aged adults
IL-21
Tetanus Toxoid
Medicine
education.field_of_study
B-Lymphocytes
Tetanus
ELISPOT
INDUCTION
Vaccination
Toxoid
T helper cell
T-Lymphocytes
Helper-Inducer
Middle Aged
Antibodies
Bacterial
PERTUSSIS
DIPHTHERIA
Infectious Diseases
medicine.anatomical_structure
DIFFERENTIATION
B-CELLS
Molecular Medicine
Receptors
CXCR5
ELDERLY ADULTS
ANTIBODY-RESPONSES
Population
T-helper cells
Conjugate-carrier vaccine
03 medical and health sciences
Interferon-gamma
Immune system
Humans
education
Aged
General Veterinary
General Immunology and Microbiology
business.industry
Interleukins
CYTOKINES
Public Health
Environmental and Occupational Health
medicine.disease
Virology
MenACWY-1T
030104 developmental biology
Immunization
T-FH-cells
Immunology
Antibody Formation
YOUNG
business
Zdroj: Vaccine, 35(42), 5581-5588. ELSEVIER SCI LTD
ISSN: 0264-410X
Popis: Introduction Vaccines frequently induce suboptimal immune responses in the elderly, due to immunological ageing. Timely vaccination may be a strategy to overcome this problem, which classifies middle-aged adults as an interesting target group for future vaccine interventions. However, the immunological fitness of the middle-aged population is ill-defined. It is currently unknown whether effective T-cell help towards B-cells is initiated by conjugate-carrier vaccines at middle-age. Aim We characterized systemic Tetanus Toxoid (TT) specific T-helper cell responses in the circulation of middle-aged adults (50–65 years of age, n = 31) having received the MenACWY-TT vaccination. Methods Blood samples were taken pre- as well as 7 days, 28 days, and 1 year post-vaccination. TT-specific T-cell responses were determined by IFNγ Elispot and by the secretion of IFNγ, IL13, IL10, IL17, and IL21 in cell culture supernatants. Circulating CD4+CXCR5+ICOS+IL21+ cells were analyzed by flow cytometry, and meningococcal and TT-specific IgG responses by bead-based immunoassays. The correlation between the T-cell help and humoral responses was evaluated. Results Vaccination with a TT-carrier protein induced a mixed TT-specific Th1 (IFNγ), Th2 (IL13, IL10), and Th17 (IL17) response in most participants. Additionally, circulating CD4+CXCR5+ICOS+IL21+ cells were significantly increased 7 days post-vaccination. Pre-vaccination TT-specific cytokine production and post-vaccination Th2 responses correlated positively with the increase of CD4+CXCR5+ICOS+IL21+ cells. No correlation between T-cell help and antibody responses was found. Conclusion The characteristics of the T-cell response upon a TT-carrier vaccination suggests effective T-cell help towards B-cells in response to meningococcal polysaccharides, although the absence of a correlation with the antibody responses warrants further clarification. However, the robust T-helper cell response in middle-aged adults, decades after previous TT vaccinations, strengthens the classification of this age group for future vaccine interventions in the context of population ageing.
Databáze: OpenAIRE
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