Form and pattern of MUC1 expression on T cells activated in vivo or in vitro suggests a function in T-cell migration
| Autor: | Tim A. Plunkett, Olivera J. Finn, Anda M. Vlad, Arron Mungul, Joyce Taylor-Papadimitriou, Jessica Candelora-Kettel, Isabel Correa, Joy Burchell |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Adult
T-Lymphocytes Immunology CD1 Gene Expression Breast Neoplasms Biology Lymphocyte Activation digestive system Arthritis Rheumatoid Interleukin 21 Cell Movement Immunology and Allergy Cytotoxic T cell Humans IL-2 receptor RNA Messenger Antigen-presenting cell skin and connective tissue diseases neoplasms Cells Cultured Interleukin 3 CD40 Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction ZAP70 Cell Membrane Mucin-1 Antibodies Monoclonal Glycosyltransferases Original Articles Molecular biology biological factors digestive system diseases Peptide Fragments Cell biology biology.protein Female |
| Popis: | MUC1 is a transmembrane mucin that is expressed on ductal epithelial cells and epithelial malignancies and has been proposed as a target antigen for immunotherapy. The expression of MUC1 has recently been reported on T and B cells. In this study we demonstrate that following activation in vivo or activation by different stimuli in vitro, human T cells expressed MUC1 at the cell surface. However, the level of expression in activated human T cells was significantly lower than that seen on normal epithelial cells or on breast cancer cells. In contrast, resting T cells did not bind MUC1-specific monoclonal antibodies (mAbs), nor was MUC1 mRNA detectable by reverse transcription-polymerase chain reaction (RT-PCR) or Northern blot analysis in these cells. The profile of activated T-cell reactivity with different MUC1-specific antibodies suggested that the glycoform of MUC1 expressed by the activated T cells carried core 2-based O-glycans, as opposed to the core 1 structures that dominate in the cancer-associated mucin. Confocal microscopy revealed that MUC1 was uniformly distributed on the surface of activated T cells. However, when the cells were polarized in response to a migratory chemokine, MUC1 was found on the leading edge rather than on the uropod, where other large mucin-like molecules on T cells are trafficked. The concentration of MUC1 at the leading edge of polarized activated human T cells suggests that MUC1 could be involved in early interactions between T cells and endothelial cells at inflammatory sites. |
| Databáze: | OpenAIRE |
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