Nuclear proteins from the rat pituitary gland bind to regulatory sequences of the thyrotrophin-beta gene
| Autor: | J. A. O. Ahlquist, D. B. Ramsden, Michael C. Sheppard, Jayne A. Franklyn |
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| Rok vydání: | 1989 |
| Předmět: |
Genetics
Regulation of gene expression Cell Nucleus Structural gene Nuclear Proteins Thyrotropin Promoter Rats Inbred Strains DNA Biology Regulatory Sequences Nucleic Acid Molecular biology Binding Competitive Rats Endocrinology Regulatory sequence Pituitary Gland Anterior Complementary DNA Gene expression Animals Electrophoresis Polyacrylamide Gel Female Nuclear protein Molecular Biology Gene |
| Zdroj: | Journal of molecular endocrinology. 2(2) |
| ISSN: | 0952-5041 |
| Popis: | Interactions between DNA sequences and nuclear proteins are important in the regulation of gene expression. We have applied a gel retardation method to examine the binding of nuclear proteins from the rat pituitary gland to regulatory sequences of the rat TSH β-subunit gene. Binding between nuclear protein and DNA is demonstrated by an alteration in electrophoretic mobility of the DNA. A 0·4 m NaCl fraction of pituitary nuclear proteins bound to a fragment of DNA containing the promoter region of the TSH-β gene, but not to plasmid DNA or insulin cDNA of comparable size. This nuclear protein fraction also bound to fragments of DNA containing sequences from the 5′-flanking region of the gene; binding of nuclear proteins was evident as far as 1 kb 5′ to the structural gene. Each of these regions of the TSH-β gene formed a number of distinct complexes with the nuclear protein extract, distinguished by their differing binding affinities in the presence of poly(dI-dC), and by their electrophoretic mobility. These findings suggest that transcriptional regulation of the rat TSH-β gene may be mediated by interaction of a number of nuclear proteins with regulatory sequences of DNA up to 1 kb upstream of the transcriptional start site of the gene. |
| Databáze: | OpenAIRE |
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