On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman
| Autor: | Shvedko, A.G., Kruglik, S.G., Ermolenkov, V.V., Orlovich, V.A., Turpin, P.Y., Greve, J., Otto, C., Greve, Jan, Puppels, Gerwin J., Otto, Cees |
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| Rok vydání: | 1999 |
| Předmět: |
Base pair
Intercalation (chemistry) Cationic polymerization chemistry.chemical_element Photochemistry Marie Curie Copper Porphyrin chemistry.chemical_compound symbols.namesake chemistry Nucleic acid symbols Stokes component Double Helix Stepanov Institute Raman spectroscopy Stimulate Raman Scattering DNA |
| Zdroj: | Spectroscopy of Biological Molecules: New Directions ISBN: 9789401059190 Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands, 265-266 STARTPAGE=265;ENDPAGE=266;TITLE=Spectroscopy of Biological Molecules: New Directions |
| DOI: | 10.1007/978-94-011-4479-7_116 |
| Popis: | Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5]. |
| Databáze: | OpenAIRE |
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