Chlorophyll biosynthesis and transcriptome profiles of chlorophyll b-deficient type 2b rice (Oryza sativa L.)
| Autor: | Szu-Hsien Lin, Hoang Chinh Nguyen, Tin-Han Shih, Giang Tuyet Pham, Chi-Ming Yang, Minh Khiem Nguyen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll b
Agriculture (General) Mutant macromolecular substances Plant Science Horticulture Photosynthesis S1-972 chemistry.chemical_compound polycyclic compounds chlorophyll Carotenoid chemistry.chemical_classification photosynthesis Oryza sativa food and beverages Forestry SD1-669.5 Photosynthetic capacity Chloroplast chemistry Biochemistry Chlorophyll gene expression transcriptome profiles Agronomy and Crop Science chl b-deficient mutant |
| Zdroj: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Vol 49, Iss 3 (2021) |
| ISSN: | 1842-4309 0255-965X |
| DOI: | 10.15835/nbha49312380 |
| Popis: | Photosynthetic and transcriptomic characteristics of a chlorophyll (Chl) b-deficient mutant type 2b rice (ch14) were investigated in this study. The ultrastructure of chloroplast in ch14 demonstrated irregular chloroplast enhancement (loss of starch granules, indistinct membranes, and thinner grana). Ch14 had significantly lower carotenoid, Chl a, Chl b, and total Chl contents, but a higher ratio of Chl a to Chl b than a wide-type rice. 3,594 genes were differentially expressed in ch14, among which 309 transcription factors were related to Chl degradation and biosynthesis, chloroplast formations, and the photosynthesis capacity. PsbR, GSA-AT, PBGD, PPOX, MgMT, and POR genes were down-regulated, reducing Chl content and photosynthetic capacity in the ch14. This study suggests that Chl degradation may be attributed to abnormal chloroplast development and down-regulation of gene expression in the common pathway and Mg branch and the rise in Chl a to Chl b ratio may be involved in the alternative Chl b degradation pathway. |
| Databáze: | OpenAIRE |
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