NOV/CCN3 induces adhesion of muscle skeletal cells and cooperates with FGF2 and IGF-1 to promote proliferation and survival
| Autor: | Cécile Martinerie, Catherine Dubois, Maryvonne Laurent, Hélène Thibout, Jérôme E. Lafont |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Integrins
Angiogenesis Cell Survival Clinical Biochemistry Integrin Apoptosis Biology Immediate-Early Proteins Myoblasts Mice Nephroblastoma Overexpressed Protein stomatognathic system Cell Adhesion Myocyte Animals Humans Insulin-Like Growth Factor I Phosphorylation Muscle Skeletal Cells Cultured Cytoskeleton Cell Proliferation Myogenesis Kinase Connective Tissue Growth Factor Cell Biology General Medicine Adhesion DNA bacterial infections and mycoses Cell biology Fibronectin Kinetics Focal Adhesion Protein-Tyrosine Kinases biology.protein Intercellular Signaling Peptides and Proteins Ectopic expression Fibroblast Growth Factor 2 Proteoglycans Mitogen-Activated Protein Kinases Poly(ADP-ribose) Polymerases |
| Zdroj: | Cell communicationadhesion. 12(1-2) |
| ISSN: | 1541-9061 |
| Popis: | During mammalian development, expression of the Nephroblastoma overexpressed gene (NOV/CCN3) is tightly regulated in skeletal muscles. Ex vivo, ectopic expression of NOV blocks myogenic differentiation. NOV also supports endothelial cell adhesion and angiogenesis through interactions with integrins. Integrins play fundamental roles during myogenesis. In this study, we show that NOV mediates adhesion and spreading of myoblasts. Myoblasts adhesion to NOV does not require proteoglycans and is dependent on integrin beta1, whereas spreading involves another RGD-sensitive integrin. The C-Terminal part of NOV as well as full-length is able to support adhesion of myoblasts; in addition, both increase focal-adhesion kinase (FAK) phosphorylation. Furthermore, NOV is an adhesive substrate that, combined with FGF2 or IGF-1, promotes cell specific proliferation and survival, respectively, in a better way than fibronectin. Taken together, these results identify NOV as an adhesion substrate for myoblasts which, in concert with growth factors, could play a role in the physiology of muscle cells. |
| Databáze: | OpenAIRE |
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