Antifungal Activities of cis-trans Citral Isomers against Trichophyton rubrum with ERG6 as a Potential Target
| Autor: | Ouyang Wuqing, Yunzhou Li, Meng-Yun Li, Yan-Hong Shang, Yin Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
dermatophytes Monoterpene 030106 microbiology Pharmaceutical Science Organic chemistry Trichophyton rubrum ergosterol biosynthesis Citral geranial Analytical Chemistry Flow cytometry Conidium 03 medical and health sciences chemistry.chemical_compound QD241-441 Drug Discovery medicine Physical and Theoretical Chemistry skin and connective tissue diseases citral Mycelium medicine.diagnostic_test biology Chemistry antifungal activity biology.organism_classification Sterol 030104 developmental biology Biochemistry Chemistry (miscellaneous) neral ERG6 Molecular Medicine Cis–trans isomerism |
| Zdroj: | Molecules, Vol 26, Iss 4263, p 4263 (2021) Molecules Volume 26 Issue 14 |
| ISSN: | 1420-3049 |
| Popis: | Trichophyton rubrum causes ringworm worldwide. Citral (CIT), extracted from Pectis plants, is a monoterpene and naturally composed of geometric isomers neral (cis-citral) and geranial (trans-citral). CIT has promising antifungal activities and ergosterol biosynthesis inhibition effects against several pathogenic fungi. However, no study has focused on neral and geranial against T. rubrum, which hinders the clinical application of CIT. This study aimed to compare antifungal activities of neral and geranial and preliminarily elucidate their ergosterol biosynthesis inhibition mechanism against T. rubrum. Herein, the disc diffusion assays, cellular leakage measurement, flow cytometry, SEM/TEM observation, sterol quantification, and sterol pattern change analyses were employed. The results showed geranial exhibited larger inhibition zones (p < 0.01 or 0.05), higher cellular leakage rates (p < 0.01), increased conidia with damaged membranes (p < 0.01) within 24 h, more distinct shriveled mycelium in SEM, prominent cellular material leakage, membrane damage, and morphological changes in TEM. Furthermore, geranial possessed more promising ergosterol biosynthesis inhibition effects than neral, and both induced the synthesis of 7-Dehydrodesmosterol and Cholesta-5,7,22,24-tetraen-3β-ol, which represented marker sterols when ERG6 was affected. These results suggest geranial is more potent than neral against T. rubrum, and both inhibit ergosterol biosynthesis by affecting ERG6. |
| Databáze: | OpenAIRE |
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