Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: Toward monitoring of mitochondrial therapy
Autor: | Egbert G. Mik, Nathalie Dollee, G.C. Schoonderwoerd, Nicolaas J. H. Raat, Mark A. Wefers Bettink, Floor A. Harms, Patricia A.C. Specht |
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Přispěvatelé: | Anesthesiology, Clinical Genetics |
Rok vydání: | 2020 |
Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Mitochondrion 03 medical and health sciences chemistry.chemical_compound Oxygen Consumption 0302 clinical medicine In vivo Animals Rats Wistar Molecular Biology Chemistry Non invasive Cell Biology Endotoxemia Pathophysiology Mitochondria Mitochondria Muscle Rats Cell biology Oxygen tension 030104 developmental biology Molecular Medicine Protoporphyrin 030217 neurology & neurosurgery Function (biology) Ex vivo |
Zdroj: | Mitochondrion, 50, 149-157. Elsevier |
ISSN: | 1567-7249 |
Popis: | Mitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO2) and oxygen disappearance rate (ODR) with the protoporphyrin IX‐triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease. LPS administration decreased mitochondrial respiration (ODR) in vivo but did not alter mitochondrial function as assessed with ex vivo techniques (high resolution respirometry and specific complex determinations). PpIX-TSLT measures in vivo mitoPO2 and ODR and can be applied non-invasively at the skin. |
Databáze: | OpenAIRE |
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