Endogenous and Exogenous Calcium Involved in the Betulin Production from Submerged Culture of Phellinus linteus Induced by Hydrogen Sulfide
| Autor: | Yaguang Zhan, Fan Guizhi, Duan Jian, Feifei Sun, Meiling Sun |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Phellinus chemistry.chemical_element Bioengineering Ethylenediaminetetraacetic acid Calcium Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound Channel blocker Chelation Hydrogen Sulfide Molecular Biology Betulin biology Basidiomycota General Medicine biology.organism_classification Triterpenes EGTA 030104 developmental biology chemistry Phellinus linteus Biotechnology Nuclear chemistry |
| Zdroj: | Applied Biochemistry and Biotechnology. 178:594-603 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | Using pharmacological and biochemical approaches, Ca(2+) involved in the betulin production in mycelia of Phellinus linteus induced by hydrogen sulfide (H2S) were investigated. The results showed that 2 mM H2S donor NaHS or 10 mM CaCl2 was found to enhance the betulin content in the mycelia of Phellinus to the maximum, which were 112.43 and 93.24% higher than that in the control, respectively. Further, NaHS and CaCl2 co-treatment also showed positive outcome, which were 128.95 or 24.52% higher than that in the control or NaHS treatment. At the same time, NaHS also enhanced the content of Ca(2+) and CaM. But, the above positive inductive effects for Ca(2+), CaM, and betulin production can be blocked with either Ca(2+) channel blocker (LaCl3, 2-aminoethoxydiphenyl borate) or Ca(2+) chelator (ethylenediaminetetraacetic acid (EDTA)). Among of them, betulin content was reduced 35.06% by NaHS and EGTA to the minimum, and this reduction could be reversed by the application of CaCl2 (NaHS + EGTA + CaCl2). From above results, it can be concluded that endogenous and exogenous calcium involved in the betulin production from submerged culture of P. linteus induced by hydrogen sulfide. |
| Databáze: | OpenAIRE |
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