A Mechanism of Resistance to HIV-1 Entry: Inefficient Interactions of CXCR4 with CD4 and gp120 in Macrophages
| Autor: | Dimiter S. Dimitrov, Yan-Ru Feng, David Norwood, Xiaodong Xiao, Tzanko S. Stantchev, Christopher C. Broder |
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| Rok vydání: | 1999 |
| Předmět: |
Receptors
CXCR4 membrane fusion envelope glycoprotein chemokine receptors HIV Envelope Protein gp120 Biology Virus Replication CXCR4 Chemokine receptor Virology Macrophage fusion Humans CCL15 Acquired Immunodeficiency Syndrome Mechanism (biology) Macrophages virus diseases Lipid bilayer fusion Immunity Innate CD4 Cell biology coreceptors Viral replication CD4 Antigens HIV-1 biology.protein Antibody HeLa Cells |
| Zdroj: | Virology. 259(1):1-6 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1999.9747 |
| Popis: | To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4–CXCR4 and gp120–CD4–CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry. |
| Databáze: | OpenAIRE |
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