Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells
| Autor: | Qingbo Li, Xiaoyan Chen, Jian Chen, Canxin Liang, Weisheng Cao, Zeng Jiang, Yu Li, Yong Xiang, Jinqun Li, Yun Yu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Small interfering RNA
Telomerase Carcinogenesis Veterinary medicine Caspase 3 Biology Virus Replication Cell Line Avian Proteins Cell Movement SF600-1100 Animals Telomerase reverse transcriptase Wnt Signaling Pathway Poultry Diseases avian leukosis virus subgroup J chicken telomerase reverse transcriptase General Veterinary Avian Leukosis Virus Cell growth Wnt signaling pathway apoptosis Wnt/β-catenin signalling pathway Cell cycle Hedgehog signaling pathway Cell biology cell proliferation Avian Leukosis Chickens Research Article |
| Zdroj: | Veterinary Research Veterinary Research, Vol 52, Iss 1, Pp 1-17 (2021) |
| ISSN: | 1297-9716 0928-4249 |
| Popis: | This study aimed to explore the mutual regulation between chicken telomerase reverse transcriptase (chTERT) and the Wnt/β-catenin signalling pathway and its effects on cell growth and avian leukosis virus subgroup J (ALV-J) replication in LMH cells. First, LMH cells stably overexpressing the chTERT gene (LMH-chTERT cells) and corresponding control cells (LMH-NC cells) were successfully constructed with a lentiviral vector expression system. The results showed that chTERT upregulated the expression of β-catenin, Cyclin D1, TCF4 and c-Myc. chTERT expression level and telomerase activity were increased when cells were treated with LiCl. When the cells were treated with ICG001 or IWP-2, the activity of the Wnt/β-catenin signalling pathway was significantly inhibited, and chTERT expression and telomerase activity were also inhibited. However, when the β-catenin gene was knocked down by small interfering RNA (siRNA), the changes in chTERT expression and telomerase activity were consistent with those in cells treated with ICG001 or IWP-2. These results indicated that chTERT and the Wnt/β-catenin signalling pathway can be mutually regulated. Subsequently, we found that chTERT not only shortened the cell cycle to promote proliferation but also inhibited apoptosis by downregulating the expression of Caspase 3, Caspase 9 and BAX; upregulating BCL-2 and BCL-X expression; and promoting autophagy. Moreover, chTERT significantly enhanced the migration ability of LMH cells, upregulated the protein and mRNA expression of ALV-J and increased the virus titre. ALV-J replication promoted chTERT expression and telomerase activity. Supplementary Information The online version contains supplementary material available at 10.1186/s13567-021-00979-x. |
| Databáze: | OpenAIRE |
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