Limitations of the GENSAT Egr1-EGFP transgenic mouse strain for neural circuit activity mapping
| Autor: | Aubrey M. Demchuk, Lilia Mesina, Sutherland Dube, Bruce L. McNaughton |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genetically modified mouse endocrine system Transgene Green Fluorescent Proteins EGR1 Gene Expression Mice Transgenic Biology Hippocampus Nervous System Green fluorescent protein 03 medical and health sciences Mice 0302 clinical medicine Genes Reporter Gene expression Animals Neural cell Gene Cerebral Cortex General Neuroscience Cell biology 030104 developmental biology Immediate early gene 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience letters. 732 |
| ISSN: | 1872-7972 |
| Popis: | Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) "Egr1-EGFP" reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein. This precautionary study outlines the limitations of the Egr1-EGFP transgenic line as a tool to study the activity-dependent expression of Egr1 and emphasizes the necessity of taking into account the potential loss of regulatory elements, stability determinants, or translational modulation in transgenic reporter strains. |
| Databáze: | OpenAIRE |
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