Identification and quantification of myosin heavy chain isoforms in bovine and porcine longissimus muscles by LC-MS/MS analysis
| Autor: | Gap-Don Kim, Kye Man Cho, Jin-Yeon Jeong, Tae-Chul Jeong |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene isoform Swine Longissimus Thoracis Peptide Tandem mass spectrometry Bioinformatics Major histocompatibility complex 03 medical and health sciences Tandem Mass Spectrometry Myosin Animals Protein Isoforms Muscle Skeletal chemistry.chemical_classification Myosin Heavy Chains biology 0402 animal and dairy science Reproducibility of Results 04 agricultural and veterinary sciences 040201 dairy & animal science Molecular biology Label-free quantification 030104 developmental biology Longissimus chemistry biology.protein Cattle Chromatography Liquid Food Science |
| Zdroj: | Meat Science. 125:143-151 |
| ISSN: | 0309-1740 |
| DOI: | 10.1016/j.meatsci.2016.12.002 |
| Popis: | To identify and quantify the myosin heavy chain (MHC) isoforms in porcine and bovine longissimus thoracis (LT) muscles, LC-MS/MS and label-free quantification were conducted. 2a, 2x, 2b and slow isoforms were identified in porcine LT muscle, whereas bovine LT muscle contained 2a, 2x and slow isoforms. The highest peak intensity (44.32%) of MHC 2b was observed in porcine LT muscle, whereas the peak intensity of MHC 2a was the highest (71.15%) in bovine LT muscle (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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