A lectin from the Chinese bird-hunting spider binds sialic acids
| Autor: | Hans-Christian, Siebert, Shan-Yun, Lu, Rainer, Wechselberger, Karin, Born, Thomas, Eckert, Songping, Liang, Claus-Wilhelm, von der Lieth, Jesús, Jiménez-Barbero, Roland, Schauer, Johannes F G, Vliegenthart, Thomas, Lütteke, Sabine, André, Herbert, Kaltner, Hans-Joachim, Gabius, Tibor, Kozár |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Models
Molecular Magnetic Resonance Spectroscopy Molecular model Molecular Sequence Data Spider Venoms Molecular modeling Model system Venom Biochemistry Mass Spectrometry Protein Structure Secondary Analytical Chemistry chemistry.chemical_compound Lectins Animals Amino Acid Sequence chemistry.chemical_classification Spider biology Organic Chemistry Lectin Spiders General Medicine Ligand (biochemistry) Sialic acid Amino acid NMR analysis chemistry Carbohydrate–protein interactions Sialic Acids biology.protein Protein Binding |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 12 páginas, 7 figuras, 1 tabla, 2 figuras suplementarias -- PAGS nros. 1515-1525 The affinity to sialic acid-containing oligosaccharides of the small-animal lectin SHL-I isolated from the venom of the Chinese bird-hunting spider Selenocosmia huwena is here described for the first time. By a strategic combination of NMR techniques, molecular modeling, and data mining tools it was possible to identify the crucial amino acid residues that are responsible for SHL-I’s ability to bind sialic acid residues in a specific way. Furthermore, we are able to discuss the role of the functional groups of sialic acid when bound to SHL-I. Also the impact of Pro31 in its cis- or trans-form on SHL-I’s ligand affinity is of special interest, since it answers the question if Trp32 is a crucial amino acid for stabilizing complexes between SHL-I and sialic acid. SHL-I can be considered as a proper model system that provides further insights into the binding mechanisms of small-animal lectins to sialic acid on a sub-molecular level The authors acknowledge Professor A. J. Olson for providing the autodock program. T. K. acknowledges the Slovak VEGA granting agency for providing support for related Project 2/7053/27 |
| Databáze: | OpenAIRE |
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